中国油脂
中國油脂
중국유지
China Oils and Fats
2015年
9期
66-69
,共4页
卢美欢%李利军%马英辉%王银存%王小娟
盧美歡%李利軍%馬英輝%王銀存%王小娟
로미환%리리군%마영휘%왕은존%왕소연
响应面法%终极腐霉%EPA%发酵工艺
響應麵法%終極腐黴%EPA%髮酵工藝
향응면법%종겁부매%EPA%발효공예
response surface methodology%Pythium ultimum%EPA%fermentation process
为进一步提高终极腐霉EPA产量,在初步优化发酵条件的基础上,通过Plackett-Burman和Box-Behnken实验设计优化终极腐霉生产 EPA 发酵工艺. 获得的最优发酵工艺条件为:蔗糖8%,硝酸钾0. 4%,酵母粉0. 65%,磷酸氢二钠0. 175%,硫酸镁0. 065%,大豆油1. 0%,起始pH 6. 0,装液量50 mL/250 mL. 在最优发酵工艺条件下,EPA产量达到541. 61 mg/L,比初步优化产量(456. 39 mg/L)提高了85. 22 mg/L.
為進一步提高終極腐黴EPA產量,在初步優化髮酵條件的基礎上,通過Plackett-Burman和Box-Behnken實驗設計優化終極腐黴生產 EPA 髮酵工藝. 穫得的最優髮酵工藝條件為:蔗糖8%,硝痠鉀0. 4%,酵母粉0. 65%,燐痠氫二鈉0. 175%,硫痠鎂0. 065%,大豆油1. 0%,起始pH 6. 0,裝液量50 mL/250 mL. 在最優髮酵工藝條件下,EPA產量達到541. 61 mg/L,比初步優化產量(456. 39 mg/L)提高瞭85. 22 mg/L.
위진일보제고종겁부매EPA산량,재초보우화발효조건적기출상,통과Plackett-Burman화Box-Behnken실험설계우화종겁부매생산 EPA 발효공예. 획득적최우발효공예조건위:자당8%,초산갑0. 4%,효모분0. 65%,린산경이납0. 175%,류산미0. 065%,대두유1. 0%,기시pH 6. 0,장액량50 mL/250 mL. 재최우발효공예조건하,EPA산량체도541. 61 mg/L,비초보우화산량(456. 39 mg/L)제고료85. 22 mg/L.
In order to increase the EPA yield produced by Pythium ultimum, the fermentation process of Pythium ultimum for EPA production was optimized by Plackett-Burman design combined with Box-Behnken design on the basis of preliminary optimal fermentation conditions. The results showed that the optimal fermentation conditions were obtained as follows: sucrose 8%, KNO3 0. 4%, yeast powder 0. 65%, Na2 HPO4 0. 175%, MgSO4 0. 065%, soybean oil 1. 0%, initial pH 6. 0, liquid volume in flask 50 mL/250 mL. Under the optimal conditions, EPA yield reached 541. 61 mg/L, which increased by 85. 22 mg/L compared with that of preliminary optimization (456. 39 mg/L) .
