CAJ | 학술논문

冷冻扫描电镜基于扫描电镜的超低温冷冻制样及传输技术发展而来，具有能在高真空状态下观察含水样品、分辨率高、制样简单快速、可对样品进行断裂刻蚀等优点，是生命科学研究的有力工具，尤其适合柔软样品的成像和监测冰冻环境对组织和细胞的影响。能否获得高质量的冷冻扫描电镜图像，取决于样品制备和观察条件是否合理和优化，要求操作人员能够快速高效地根据样品选择操作条件、捕捉快速变化的样品表面信息。
냉동소묘전경기우소묘전경적초저온냉동제양급전수기술발전이래，구유능재고진공상태하관찰함수양품、분변솔고、제양간단쾌속、가대양품진행단렬각식등우점，시생명과학연구적유력공구，우기괄합유연양품적성상화감측빙동배경대조직화세포적영향。능부획득고질량적냉동소묘전경도상，취결우양품제비화관찰조건시부합리화우화，요구조작인원능구쾌속고효지근거양품선택조작조건、포착쾌속변화적양품표면신식。
Cryo?scanning electron microscopy ( Cryo?SEM ) , which combines the high?performance imaging of scanning electron microscopy ( SEM) with cryogenic sample preparation techniques, allows scientists to investigate structures and materials in their native, hydrated state with image resolution now approaching 1 nm. Cryo?SEM is a powerful tool for characterizing the hydrated microstructures of biological specimens, especially for observing soft specimens ( like tissues and cells) and their responses to freezing. Operator skill is the primary factor in determining the quality of the results. The operator’ s ability to quickly and efficiently scan the surface, manipulate conditions, and acquire images of the rapidly changing sample are very important.