中国现代中药
中國現代中藥
중국현대중약
Modern Chinese Medicine
2015年
9期
905-910
,共6页
张文娟%尚柯%魏锋%马双成
張文娟%尚柯%魏鋒%馬雙成
장문연%상가%위봉%마쌍성
太白贝母%伊犁贝母%鉴别%性状%聚合酶链式反应-限制性片段长度多态性方法(PCR-RFLP)%内转录间隔区(ITS)
太白貝母%伊犛貝母%鑒彆%性狀%聚閤酶鏈式反應-限製性片段長度多態性方法(PCR-RFLP)%內轉錄間隔區(ITS)
태백패모%이리패모%감별%성상%취합매련식반응-한제성편단장도다태성방법(PCR-RFLP)%내전록간격구(ITS)
Fritillaria taipaiensis%F. pallidiflora%identification%characteristics%polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP)%internal transcribed spacer (ITS)
目的：探讨聚合酶链式反应-限制性片段长度多态性方法（PCR-RFLP）对于太白贝母的适用性。方法：采集太白贝母野生及栽培品，收集市场上太白贝母伪品，利用性状、PCR-RFLP 鉴别法、DNA 序列分析三种方法分析太白贝母与常见伪品的区别。结果：从性状上看，太白贝母栽培品与伪品往往非常相似，较难辨别；通过 PCR-RFLP 方法可以正确、客观的鉴别太白贝母与常见伪品，基于 ITS2的 DNA 条形码方法进一步验证了 PCR-RFLP 法的准确性。市场上太白贝母的伪品多为伊犁贝母。结论：PCR-RFLP 鉴别法可准确鉴别太白贝母与其伪品；市场上太白贝母伪品较多，应加强监管。
目的：探討聚閤酶鏈式反應-限製性片段長度多態性方法（PCR-RFLP）對于太白貝母的適用性。方法：採集太白貝母野生及栽培品，收集市場上太白貝母偽品，利用性狀、PCR-RFLP 鑒彆法、DNA 序列分析三種方法分析太白貝母與常見偽品的區彆。結果：從性狀上看，太白貝母栽培品與偽品往往非常相似，較難辨彆；通過 PCR-RFLP 方法可以正確、客觀的鑒彆太白貝母與常見偽品，基于 ITS2的 DNA 條形碼方法進一步驗證瞭 PCR-RFLP 法的準確性。市場上太白貝母的偽品多為伊犛貝母。結論：PCR-RFLP 鑒彆法可準確鑒彆太白貝母與其偽品；市場上太白貝母偽品較多，應加彊鑑管。
목적：탐토취합매련식반응-한제성편단장도다태성방법（PCR-RFLP）대우태백패모적괄용성。방법：채집태백패모야생급재배품，수집시장상태백패모위품，이용성상、PCR-RFLP 감별법、DNA 서렬분석삼충방법분석태백패모여상견위품적구별。결과：종성상상간，태백패모재배품여위품왕왕비상상사，교난변별；통과 PCR-RFLP 방법가이정학、객관적감별태백패모여상견위품，기우 ITS2적 DNA 조형마방법진일보험증료 PCR-RFLP 법적준학성。시장상태백패모적위품다위이리패모。결론：PCR-RFLP 감별법가준학감별태백패모여기위품；시장상태백패모위품교다，응가강감관。
[Abstract ] Objective: To explore the applicability of polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP)on identification of Fritillaria taipaiensis Bulbus.Methods:The samples of wild and cultivated F.taipaiensis and the samples from the market were colleeted,and they were identified from the common adulterants adulterants based on characteristics,PCR-RFLP,and DNA sequence analysis.Results:It was difficult to identify F.taipaiensis from its adulterants by characteristics as they showed similar appearance.With the method of PCR-RFLP F.taipaiensis was accurately and objectively identified,which was further verified by DNA barcoding based on ITS2.On the market,most of the adulterants of F.taipaiensis were F.pallidiflora.Conclusion:The method of PCR-RFLP could be successfully applied in the identification of F.taipaiensis.The adulterants of F.taipaiensis on the market are so common that its quality control needs to be strengthened.
