CAJ | 학술논문

该文以中碳链甘三酯和大豆油为原料，对酶法酯交换催化合成中/长链结构甘三酯的工艺进行研究。系统研究了脂肪酶的种类、脂肪酶添加量、反应温度和反应时间四个因素对酯交换反应程度的影响，结果表明，选用脂肪酶TL IM，脂肪酶添加量5%（以油重计），反应温度65℃，反应时间30 min时酯交换反应即达到平衡状态，中/长链结构甘三酯的含量为73.73%，符合结构甘油三酯进口药品注册标准的含量要求。正交极差分析结果显示，选定脂肪酶的添加量是影响酯交换反应程度最重要的因素，反应温度次之，反应时间最小。
해문이중탄련감삼지화대두유위원료，대매법지교환최화합성중/장련결구감삼지적공예진행연구。계통연구료지방매적충류、지방매첨가량、반응온도화반응시간사개인소대지교환반응정도적영향，결과표명，선용지방매TL IM，지방매첨가량5%（이유중계），반응온도65℃，반응시간30 min시지교환반응즉체도평형상태，중/장련결구감삼지적함량위73.73%，부합결구감유삼지진구약품주책표준적함량요구。정교겁차분석결과현시，선정지방매적첨가량시영향지교환반응정도최중요적인소，반응온도차지，반응시간최소。
Basedon themedium-chain triglycerides and soybeanoil as rawmaterial, the enzymatic transesterificationcatalyzed synthesis themedium/long-chain structure triglycerides process was studied in the paper.The influencesof the kindsof lipase, lipase load, reaction temperature and timeon the degreeof transesterification were systematically studied, and the results showed that theLipozyme TL IM, 5% lipase/oil(w/w), reaction temperature 65℃, reaction time 30min were theoptimal conditions and thecontentofmedium/long-chain structureof triglycerides was 73.73%, which was in line with the imported drug registration standard requirementsof structure triglycerides. We also found that the amountofLipozymeTL RM was themost important factor for the degreeof transesterification by theorthogonal range analysis, followed by the reaction temperature and reaction time.