亚太热带医药杂志（英文版）
亞太熱帶醫藥雜誌（英文版）
아태열대의약잡지（영문판）
Asian pacific Journal of Tropical Medicine
2014年
11期
854-859
,共6页
Trypanosoma cruzi%Polymerase chain reaction%Colombia%Superoxide dismutase gene b-based primers%Discrete typing unit
Objective:To classify 21 new isolates of Trypanosoma cruzi (T. cruzi) according to the Discrete Typing Unit (DTU) which they belong to, as well as tune up a new pair of primers designed to detect the parasite in biological samples. Methods: Strains were isolated, DNA extracted, and classified by using three Polymerase Chain Reactions (PCR). Subsequently this DNA was used along with other isolates of various biological samples, for a new PCR using primers designed. Finally, the amplified fragments were sequenced. Results: It was observed the predominance of DTU I in Colombia, as well as the specificity of our primers for detection of T. cruzi, while no band was obtained when other species were used. Conclusions:This work reveals the genetic variability of 21 new isolates of T. cruzi in Colombia.Our primers confirmed their specificity for detecting the presence of T. cruzi.
