CAJ | 학술논문

目的：明确金银花上的棉铃虫、金银花尺蠖2种害虫的病原真菌的分类地位，为寻找对药材鳞翅目害虫有高毒力的病原真菌提供材料。方法：通过分生孢子分离、培养，从受真菌侵染死亡的金银花鳞翅目害虫体表分离获得昆虫病原真菌，依据真菌的形态特征和 ITS 序列特征对其进行鉴定。结果：从12份受真菌感染死亡的幼虫体表分离到12株形态特征一致的真菌，依据形态特征和 ITS 序列特征将其鉴定为莱氏野村菌 Nomurea rileyi。结论：莱氏野村菌为金银花尺蠖及棉铃虫两种金银花鳞翅目害虫的一种病原真菌，因其能够在某些鳞翅目害虫间形成流行病，对防治药材害虫具有应用潜力。
목적：명학금은화상적면령충、금은화척확2충해충적병원진균적분류지위，위심조대약재린시목해충유고독력적병원진균제공재료。방법：통과분생포자분리、배양，종수진균침염사망적금은화린시목해충체표분리획득곤충병원진균，의거진균적형태특정화 ITS 서렬특정대기진행감정。결과：종12빈수진균감염사망적유충체표분리도12주형태특정일치적진균，의거형태특정화 ITS 서렬특정장기감정위래씨야촌균 Nomurea rileyi。결론：래씨야촌균위금은화척확급면령충량충금은화린시목해충적일충병원진균，인기능구재모사린시목해충간형성류행병，대방치약재해충구유응용잠력。
Objective: To identify the entomopathogenic fungi which killing Heterolocha jinyinhuaphaga and Helicoverpa armigera damaging Lonicera japonica Thunb.Methods:Eleven samples of Heterolocha jinyinhuaphaga and one sample of Helicoverpa armigera lepidoptera larvae infected by entomopathogenic fungi were collected from Lonicera japonica Thunb,twelve entomopathogenic fungi strains were isolated by culturing spores washing from dead larvae,these fungi were identified with morphological characteristic and rDNA ITS sequence.Results:All strains of fungi were identified as Nomurea rileyi.Conclusion:Nomurea rileyi is one kind of entomopathogenic fungus that could kill some kind of lepidoptera larvae on the medicinal plant of Lonicera japonica Thunb,it will be useful research materials for study to control insects of Lonicera japonica Thunb and other medicinal plants.