中国现代中药
中國現代中藥
중국현대중약
Modern Chinese Medicine
2015年
9期
931-933
,共3页
单会娇%王冰%张建逵%许亮
單會嬌%王冰%張建逵%許亮
단회교%왕빙%장건규%허량
龙葵%澳洲茄碱%HPLC%药用部位%生长周期%成熟程度
龍葵%澳洲茄堿%HPLC%藥用部位%生長週期%成熟程度
룡규%오주가감%HPLC%약용부위%생장주기%성숙정도
Solanum nigrum%solasonine%HPLC%medicinal parts%growth cycle%maturity
目的：通过研究龙葵不同产地、生长周期及药用部位中澳洲茄碱的动态规律，为进一步优化龙葵的采收期、药用部位及栽培提供依据。方法：采用 HPLC，AgilentTC-C18色谱柱（250 mm ×4.6 mm）；以乙腈-0.5％磷酸水溶液作为流动相，梯度洗脱；检测波长为203 nm；体积流量：0.9 mL·min －1；柱温为30℃。结果：龙葵药材中澳洲茄碱含量因产地不同差异很大。龙葵地上部分澳洲茄碱含量的高低主要取决于所结果实的多少，以盛果期含量最高；不同成熟程度果实中澳洲茄碱含量的规律为：成熟果实＜青果＜幼果。结论：在龙葵的生长过程中，龙葵中澳洲茄碱的含量随着其生长发育进程而发生变化。根据龙葵不同生长阶段所含澳洲茄碱在各器官中的含量变化，龙葵的最佳采收期为盛果期，最佳药用部位为幼果和青果。
目的：通過研究龍葵不同產地、生長週期及藥用部位中澳洲茄堿的動態規律，為進一步優化龍葵的採收期、藥用部位及栽培提供依據。方法：採用 HPLC，AgilentTC-C18色譜柱（250 mm ×4.6 mm）；以乙腈-0.5％燐痠水溶液作為流動相，梯度洗脫；檢測波長為203 nm；體積流量：0.9 mL·min －1；柱溫為30℃。結果：龍葵藥材中澳洲茄堿含量因產地不同差異很大。龍葵地上部分澳洲茄堿含量的高低主要取決于所結果實的多少，以盛果期含量最高；不同成熟程度果實中澳洲茄堿含量的規律為：成熟果實＜青果＜幼果。結論：在龍葵的生長過程中，龍葵中澳洲茄堿的含量隨著其生長髮育進程而髮生變化。根據龍葵不同生長階段所含澳洲茄堿在各器官中的含量變化，龍葵的最佳採收期為盛果期，最佳藥用部位為幼果和青果。
목적：통과연구룡규불동산지、생장주기급약용부위중오주가감적동태규률，위진일보우화룡규적채수기、약용부위급재배제공의거。방법：채용 HPLC，AgilentTC-C18색보주（250 mm ×4.6 mm）；이을정-0.5％린산수용액작위류동상，제도세탈；검측파장위203 nm；체적류량：0.9 mL·min －1；주온위30℃。결과：룡규약재중오주가감함량인산지불동차이흔대。룡규지상부분오주가감함량적고저주요취결우소결과실적다소，이성과기함량최고；불동성숙정도과실중오주가감함량적규률위：성숙과실＜청과＜유과。결론：재룡규적생장과정중，룡규중오주가감적함량수착기생장발육진정이발생변화。근거룡규불동생장계단소함오주가감재각기관중적함량변화，룡규적최가채수기위성과기，최가약용부위위유과화청과。
Objective:To the dynamic accumulation of solasonine in Solanum nigrum from different origin growth cycle and medicinal parts,and provide scientific basis for harvest time,standardized cultivation and medicinal parts of S.nigrum.Methods:The content of solasonine was determined by using HPLC,Agilent TC-C18 column (250 mm ×4.6 mm) with acetonitrile-0.5% phosphoric acid solution as mobile phase, detection wavelength was 203 nm; flow rate was 0.9 mL·min-1 ;column temperature was 30 ℃.Results:Solasonine content of S.nigrum in great difference by different origin.The content of solasonine in S.nigrum depended on the number of the fruits.The highest content was in full fruit period.The solasonine content in fruits of different maturity was following: mature fruit < green fruit < young fruit.Conclusion:In the whole process of the growth cycle of S.nigrum,the content of solasonine in S.nigrum changed in response to its growth and development process.Based on the data that the contents of solasonine in S.nigrum changing according to different growth stages,It is concluded that the best collection period would be full bearing period,the best medicinal part would be young fruits and green fruits.
