CAJ | 학술논문

利用高效液相色谱法（HPLC）测定谷氨酰胺含量，对应用胰蛋白酶和Protamex复合酶双酶组合水解豆粕蛋白制备谷氨酰胺结合肽（Gln）的组成和贮藏稳定性进行研究。将谷氨酰胺肽进行分子量分布测定、不同贮藏条件下贮藏稳定性试验，以及85℃、6周的加速试验。研究结果表明，谷氨酰胺肽初产物中分子量分布大部分在1000 Da以下，约70%集中在500 Da~132 Da之间，只有不到10%分布在132 Da以下，谷氨酰胺的含量是6.9387%；粉末4℃和室温（25℃）、溶液4℃和室温（25℃）恒湿密封贮藏，Gln的水解率分别为2.8709%、3.1164%、3.2649%、3.3097%；经过85℃、6周的加速试验，Gln的水解率为8.1561%。
이용고효액상색보법（HPLC）측정곡안선알함량，대응용이단백매화Protamex복합매쌍매조합수해두박단백제비곡안선알결합태（Gln）적조성화저장은정성진행연구。장곡안선알태진행분자량분포측정、불동저장조건하저장은정성시험，이급85℃、6주적가속시험。연구결과표명，곡안선알태초산물중분자량분포대부분재1000 Da이하，약70%집중재500 Da~132 Da지간，지유불도10%분포재132 Da이하，곡안선알적함량시6.9387%；분말4℃화실온（25℃）、용액4℃화실온（25℃）항습밀봉저장，Gln적수해솔분별위2.8709%、3.1164%、3.2649%、3.3097%；경과85℃、6주적가속시험，Gln적수해솔위8.1561%。
Thecontentof glutamine was analyzed byHPLC,and thecomponents and stabilityof Gln from soybeanmeal protein byhydrolysisof trypsinase andProtamex were studied.Themolecular weight distributionof glutamine peptide, the storage stability testof glutamine peptideon differentconditions and 85℃ accelerated test for 6 weeks were tested.The results showed thatmostof themolecular weight distributionconcentrated under 1 000 Da, about 70%concentrated between 500 Da~132 Da andonly less than 10% distributed below 132 Da.The Glncontent was 6.938 7%.Thehydrolysis rateof Gln which was at 4℃ and 25℃ for power,and 4℃ and 25℃ for liquid was 2.870 9%, 3.116 4%,3.264 9%,3.309 7%,respectively.Thehydrolysis rateof Gln was 8.156 1% after 85℃accelerated test for 6 weeks.