中国现代中药
中國現代中藥
중국현대중약
Modern Chinese Medicine
2015年
9期
962-964
,共3页
唐炳兰%莫单丹%袁经权%刘振杰%周小雷
唐炳蘭%莫單丹%袁經權%劉振傑%週小雷
당병란%막단단%원경권%류진걸%주소뢰
苦石莲胶囊%Neocaesalpin L%含量测定%高效液相
苦石蓮膠囊%Neocaesalpin L%含量測定%高效液相
고석련효낭%Neocaesalpin L%함량측정%고효액상
Caesalpinia minax capsule%Neocaesalpin L%content determination%HPLC
目的：建立测定苦石莲胶囊中 Neocaesalpin L 含量的反相高效液相色谱法。方法：色谱柱为伊力特ODS C18（250 mm ×4.6 mm，5μm）；流动相为乙腈-0.5％磷酸水梯度洗脱；检测波长为210 nm；柱温为30℃；流速为0.8 mL·min －1。结果：Neocaesalpin L 在0.74～3.16μg 线性关系良好，r ＝0.9999，Neocaesalpin L 平均回收率为96.76％，RSD ＝1.59％（n ＝9）。结论：该方法简单，专属性强，重复性好，可有效控制苦石莲胶囊的质量。
目的：建立測定苦石蓮膠囊中 Neocaesalpin L 含量的反相高效液相色譜法。方法：色譜柱為伊力特ODS C18（250 mm ×4.6 mm，5μm）；流動相為乙腈-0.5％燐痠水梯度洗脫；檢測波長為210 nm；柱溫為30℃；流速為0.8 mL·min －1。結果：Neocaesalpin L 在0.74～3.16μg 線性關繫良好，r ＝0.9999，Neocaesalpin L 平均迴收率為96.76％，RSD ＝1.59％（n ＝9）。結論：該方法簡單，專屬性彊，重複性好，可有效控製苦石蓮膠囊的質量。
목적：건립측정고석련효낭중 Neocaesalpin L 함량적반상고효액상색보법。방법：색보주위이력특ODS C18（250 mm ×4.6 mm，5μm）；류동상위을정-0.5％린산수제도세탈；검측파장위210 nm；주온위30℃；류속위0.8 mL·min －1。결과：Neocaesalpin L 재0.74～3.16μg 선성관계량호，r ＝0.9999，Neocaesalpin L 평균회수솔위96.76％，RSD ＝1.59％（n ＝9）。결론：해방법간단，전속성강，중복성호，가유효공제고석련효낭적질량。
Objective:To establish an RP-HPLC method for quantitative determination of neocaesalpin L in Caesalpinia minax capsule.Methods:The separation was performed on a Elite ODS C18 Column (250 mm ×4.6 mm,5 μm) acetonitril-0.5% H3 PO4 was used as the mobile phase.The detection wavelength was at 210 nm.The temperature was maintained at 30 ℃.The flow rate was 0.8 mL·min -1 .Results:The concentration of neocaesalpin L was linear within the range of 0.74 -3.16 μg (r =0.999 9).The average recovery and RSD of the method were 96.76% and 1.59% (n =9). Conclusion:The method is accurate,specific,reproducible which can effectively be used in quality control of neocaesalpin L in Caesalpinia minax capsule.
