中华医学杂志
中華醫學雜誌
중화의학잡지
National Medical Journal of China
2015年
37期
3050-3053
,共4页
周蕾%张慧杰%黄成琳%庞晓奡%张淑兰
週蕾%張慧傑%黃成琳%龐曉奡%張淑蘭
주뢰%장혜걸%황성림%방효오%장숙란
死亡结构域相关蛋白%HPV16 E6%宫颈病变%免疫组织化学
死亡結構域相關蛋白%HPV16 E6%宮頸病變%免疫組織化學
사망결구역상관단백%HPV16 E6%궁경병변%면역조직화학
Death domain associated protein%Human papillomavirus 16 E6%Uterine cervical diseases%Immunohistochemistry
目的 探讨Daxx与人乳头瘤病毒(HPV) 16 E6在宫颈病变中的表达情况及其意义.方法 对2010年9月至2013年11月就诊于中国医科大学附属盛京医院妇产科门诊活检或手术切除宫颈组织标本[其中宫颈癌63例,宫颈上皮不典型增生(CIN)Ⅲ44例,Ⅰ～Ⅱ45例,正常宫颈标本42例],采用免疫组化SABC法,检测Daxx及HPV16 F6的表达情况.结果 (1)在慢性宫颈炎、CINⅠ～Ⅱ、CINⅢ和宫颈癌中Daxx的阳性表达率分别为28.57％(12/42),40.00％(18/45),65.91％(29/44),66.67％ (42/63),HPV16 E6为15.38％(6/39),36.17％(17/47),46.30％(25/54),100.00％(24/24);二者在宫颈高度病变组表达高于低级别组(P＜0.05).(2) HPV16 F6高表达组的Daxx表达率高于HPV16 E6低表达组(P＜0.05).(3) Daxx的表达与宫颈癌的病理分级、临床分期、淋巴结转移情况及患者年龄无关(P＞0.05).(4) Daxx与HPV16 E6的阳性表达呈正相关(r=0.695,P＜0.05).结论 Daxx和HPV16 E6随着宫颈病变级别的升高表达增加,两者可能存在某种协同作用,可能对宫颈病变发展起促进作用.
目的 探討Daxx與人乳頭瘤病毒(HPV) 16 E6在宮頸病變中的錶達情況及其意義.方法 對2010年9月至2013年11月就診于中國醫科大學附屬盛京醫院婦產科門診活檢或手術切除宮頸組織標本[其中宮頸癌63例,宮頸上皮不典型增生(CIN)Ⅲ44例,Ⅰ～Ⅱ45例,正常宮頸標本42例],採用免疫組化SABC法,檢測Daxx及HPV16 F6的錶達情況.結果 (1)在慢性宮頸炎、CINⅠ～Ⅱ、CINⅢ和宮頸癌中Daxx的暘性錶達率分彆為28.57％(12/42),40.00％(18/45),65.91％(29/44),66.67％ (42/63),HPV16 E6為15.38％(6/39),36.17％(17/47),46.30％(25/54),100.00％(24/24);二者在宮頸高度病變組錶達高于低級彆組(P＜0.05).(2) HPV16 F6高錶達組的Daxx錶達率高于HPV16 E6低錶達組(P＜0.05).(3) Daxx的錶達與宮頸癌的病理分級、臨床分期、淋巴結轉移情況及患者年齡無關(P＞0.05).(4) Daxx與HPV16 E6的暘性錶達呈正相關(r=0.695,P＜0.05).結論 Daxx和HPV16 E6隨著宮頸病變級彆的升高錶達增加,兩者可能存在某種協同作用,可能對宮頸病變髮展起促進作用.
목적 탐토Daxx여인유두류병독(HPV) 16 E6재궁경병변중적표체정황급기의의.방법 대2010년9월지2013년11월취진우중국의과대학부속성경의원부산과문진활검혹수술절제궁경조직표본[기중궁경암63례,궁경상피불전형증생(CIN)Ⅲ44례,Ⅰ～Ⅱ45례,정상궁경표본42례],채용면역조화SABC법,검측Daxx급HPV16 F6적표체정황.결과 (1)재만성궁경염、CINⅠ～Ⅱ、CINⅢ화궁경암중Daxx적양성표체솔분별위28.57％(12/42),40.00％(18/45),65.91％(29/44),66.67％ (42/63),HPV16 E6위15.38％(6/39),36.17％(17/47),46.30％(25/54),100.00％(24/24);이자재궁경고도병변조표체고우저급별조(P＜0.05).(2) HPV16 F6고표체조적Daxx표체솔고우HPV16 E6저표체조(P＜0.05).(3) Daxx적표체여궁경암적병리분급、림상분기、림파결전이정황급환자년령무관(P＞0.05).(4) Daxx여HPV16 E6적양성표체정정상관(r=0.695,P＜0.05).결론 Daxx화HPV16 E6수착궁경병변급별적승고표체증가,량자가능존재모충협동작용,가능대궁경병변발전기촉진작용.
Objective To investigate the expression of death domain associated protein (Daxx) and human papillomavirus 16 E6 protein (HPV16 E6) in cervical lesions and analyze their significance.Methods Immunohistochemical SABC method was used to detect the expression of Daxx and HPV16 E6 in 194 cases of cervical tissues with different lesions.Results (1) The positive expression rate of Daxx was 28.57％ (12/42),40.00％ (18/45),65.91％ (29/44),66.67％ (42/63),respectively,in chronic cervicitis,cervical intraepithelial neoplasia Ⅰ-Ⅱ (CIN Ⅰ-Ⅱ),CIN Ⅲ and cervical squamous cell carcinoma.The positive expression rate of HPV16 E6 was 15.38％ (6/39),36.17％ (17/47),46.30％ (25/54),100.00％ (24/24) in the above four groups.The positive rates in cervical cancer group and high grade CIN group were significantly higher than these in low level of CIN group and chronic cervicitis group (P ＜0.05).(2) The expression of Daxx was stronger in HPV16 E6 high positive group than that in HPV16 E6 low positive group (P ＜ 0.05).(3) There was no significant relationship between the expression of Daxx and pathological classification,clinical stage,situation of lymph node metastasis or patients' age in cervical squamous cell carcinoma (P ＞ 0.05).(4) There was significant positive relationship between Daxx and HPV16 E6 expression (r =0.695,P ＜ 0.05).Conclusions The expression of Daxx and HPV16 E6 gradually increases with cervical lesion degree aggravating.Here might be synergy between them,and both could promote the development of cervical lesions.
