中国综合临床
中國綜閤臨床
중국종합림상
Clinical Medicine of China
2015年
10期
865-867
,共3页
张晚鱼%王林%米源%王雷
張晚魚%王林%米源%王雷
장만어%왕림%미원%왕뢰
食管鳞癌%Hedgehog信号通路%转录因子Gli1%免疫组织化学
食管鱗癌%Hedgehog信號通路%轉錄因子Gli1%免疫組織化學
식관린암%Hedgehog신호통로%전록인자Gli1%면역조직화학
Esophageal squamous cell carcinoma%Hedgehog signal pathway%Gli1 transcription factor%Immunohistochemistry
目的 探讨Hedgehog(Hh)信号通路中Shh和Gli1与食管鳞状细胞癌的关系.方法 应用免疫组积化学SP法检测食管鳞癌组织(n=64)和正常食管黏膜组织(n=24)中Shh和Gli1蛋白的表达情况.结果 食管鳞癌组织中Shh和Gli1的阳性表达率均显著高于正常食管黏膜组织[67.19％(43/64)与4.17％ (1/24),60.94％(39/64)与4.17％ (1/24)],差异均有统计学意义(x2值分别为27.729、22.689,P均＜0.01).Shh蛋白的表达在食管鳞癌细胞的不同分化组、不同临床分期组间及有无淋巴结转移组差异均有统计学意义(x2值分别为3.873、11.349和6.429,P＜0.05或P＜0.01);Gli1表达率在食管鳞癌细胞的不同分化组、不同临床分期组间及有无淋巴结转移组差异均有统计学意义(x2值分别为12.598、9.741和26.341,P均＜0.01);食管鳞癌组织中Shh与Gli1表达呈显著正相关(r=0.259,P＜0.05).结论 Hh信号通路在食管鳞癌组织中异常活化,Shh、Gli1表达与食管鳞癌发生发展及侵袭转移有关,可能成为新的治疗食管鳞癌的靶点.
目的 探討Hedgehog(Hh)信號通路中Shh和Gli1與食管鱗狀細胞癌的關繫.方法 應用免疫組積化學SP法檢測食管鱗癌組織(n=64)和正常食管黏膜組織(n=24)中Shh和Gli1蛋白的錶達情況.結果 食管鱗癌組織中Shh和Gli1的暘性錶達率均顯著高于正常食管黏膜組織[67.19％(43/64)與4.17％ (1/24),60.94％(39/64)與4.17％ (1/24)],差異均有統計學意義(x2值分彆為27.729、22.689,P均＜0.01).Shh蛋白的錶達在食管鱗癌細胞的不同分化組、不同臨床分期組間及有無淋巴結轉移組差異均有統計學意義(x2值分彆為3.873、11.349和6.429,P＜0.05或P＜0.01);Gli1錶達率在食管鱗癌細胞的不同分化組、不同臨床分期組間及有無淋巴結轉移組差異均有統計學意義(x2值分彆為12.598、9.741和26.341,P均＜0.01);食管鱗癌組織中Shh與Gli1錶達呈顯著正相關(r=0.259,P＜0.05).結論 Hh信號通路在食管鱗癌組織中異常活化,Shh、Gli1錶達與食管鱗癌髮生髮展及侵襲轉移有關,可能成為新的治療食管鱗癌的靶點.
목적 탐토Hedgehog(Hh)신호통로중Shh화Gli1여식관린상세포암적관계.방법 응용면역조적화학SP법검측식관린암조직(n=64)화정상식관점막조직(n=24)중Shh화Gli1단백적표체정황.결과 식관린암조직중Shh화Gli1적양성표체솔균현저고우정상식관점막조직[67.19％(43/64)여4.17％ (1/24),60.94％(39/64)여4.17％ (1/24)],차이균유통계학의의(x2치분별위27.729、22.689,P균＜0.01).Shh단백적표체재식관린암세포적불동분화조、불동림상분기조간급유무림파결전이조차이균유통계학의의(x2치분별위3.873、11.349화6.429,P＜0.05혹P＜0.01);Gli1표체솔재식관린암세포적불동분화조、불동림상분기조간급유무림파결전이조차이균유통계학의의(x2치분별위12.598、9.741화26.341,P균＜0.01);식관린암조직중Shh여Gli1표체정현저정상관(r=0.259,P＜0.05).결론 Hh신호통로재식관린암조직중이상활화,Shh、Gli1표체여식관린암발생발전급침습전이유관,가능성위신적치료식관린암적파점.
Objective To study the relationship between expression of Shh and Gli1 in Hedgehog(Hh) signal pathway and esophageal squamous cell carcinoma (ESCC).Methods Expressions of Shh andGli1 were detected in 64 cases primary tumor tissues and 24 cases normal esophageal mucosa with SP immunohistochemistal method.Results There were significant differences of Shh and Gli1 expressions between esophageal carcinoma and normal mucosa epithelium (67.19％ (43/64) vs.4.17％ (1/24), 60.94％ (39/64) vs.4.17％ (1/24);x2 =27.729,22.689;P＜0.01).There were significant differences of Shh expression between different degrees of differentiation in esophageal squamous cell carcinoma, metastasis of lymph node and different clinical staging group (x2 =3.873, 11.349,6.429;P ＜ 0.05 or P＜ 0.01).The positive expression rate of Gli1 showed significant differences between different degrees of differentiation in esophageal squamous cell carcinoma, metastasis of lymph node and different clinical staging group (x2=12.598, 9.741,26.341;P ＜0.01).There was a positive relationship between Shh and Gli1 expression(r =0.259, P＜0.05).Conclusion Hh signaling pathway is abnormally activated in ESCC, so that Shh and Gli1 play important roles in carcinogenesis and progression ofesophageal carcinoma.The Hh signaling pathway may be a useful target in ESCC.
