大连民族学院学报
大連民族學院學報
대련민족학원학보
Journal of Dalian Nationalities University
2015年
5期
453-457
,共5页
李兴太%韦豪华%张红玲%孙海波%苏亚茹
李興太%韋豪華%張紅玲%孫海波%囌亞茹
리흥태%위호화%장홍령%손해파%소아여
芪参补气药茶(ACT)%黄芪%党参%线粒体活性氧
芪參補氣藥茶(ACT)%黃芪%黨參%線粒體活性氧
기삼보기약다(ACT)%황기%당삼%선립체활성양
Astragalus membranaceus/Codonopsis pilosula Qi-invigorating herbal tea(ACT)%Astragalus membranaceus%Codonopsis pilosula%mitochondrial reactive oxygen species
以黄芪和党参为原料,根据中医学原理组方制成芪参补气药茶(ACT),探索ACT清除线粒体活性氧作用及促进健康的机制. 分别用AlCl3 比色法和硫酸苯酚法测定ACT的功能因子总黄酮、总糖及总多糖含量. 以还原型辅酶Ⅰ/吩嗪硫酸甲酯( NADH/PMS)为超氧阴离子( O2 ·-)生成系统,过氧化氢( H2 O2 )/Fe2+体系为羟自由基( ·OH)生成系统,分别用氮蓝四唑( NBT)还原法和Fenton反应显色法测定ACT清除O2 ·-及·OH的能力;用Na2 S2 O3 滴定法测定ACT清除H2 O2 的能力. ACT总糖质量分数为(26 ±1.3) mg·mL-1,总多糖质量分数为(12.5 ±0.8) mg·mL-1,总黄酮质量分数为(121 ±8.5) μg ·mL-1. ACT可一定程度上清除O2 ·-、·OH和H2 O2. ACT具有温和的抗氧化作用,从而维持机体氧化与抗氧化平衡,这是ACT促进健康的潜在机制.
以黃芪和黨參為原料,根據中醫學原理組方製成芪參補氣藥茶(ACT),探索ACT清除線粒體活性氧作用及促進健康的機製. 分彆用AlCl3 比色法和硫痠苯酚法測定ACT的功能因子總黃酮、總糖及總多糖含量. 以還原型輔酶Ⅰ/吩嗪硫痠甲酯( NADH/PMS)為超氧陰離子( O2 ·-)生成繫統,過氧化氫( H2 O2 )/Fe2+體繫為羥自由基( ·OH)生成繫統,分彆用氮藍四唑( NBT)還原法和Fenton反應顯色法測定ACT清除O2 ·-及·OH的能力;用Na2 S2 O3 滴定法測定ACT清除H2 O2 的能力. ACT總糖質量分數為(26 ±1.3) mg·mL-1,總多糖質量分數為(12.5 ±0.8) mg·mL-1,總黃酮質量分數為(121 ±8.5) μg ·mL-1. ACT可一定程度上清除O2 ·-、·OH和H2 O2. ACT具有溫和的抗氧化作用,從而維持機體氧化與抗氧化平衡,這是ACT促進健康的潛在機製.
이황기화당삼위원료,근거중의학원리조방제성기삼보기약다(ACT),탐색ACT청제선립체활성양작용급촉진건강적궤제. 분별용AlCl3 비색법화류산분분법측정ACT적공능인자총황동、총당급총다당함량. 이환원형보매Ⅰ/분진류산갑지( NADH/PMS)위초양음리자( O2 ·-)생성계통,과양화경( H2 O2 )/Fe2+체계위간자유기( ·OH)생성계통,분별용담람사서( NBT)환원법화Fenton반응현색법측정ACT청제O2 ·-급·OH적능력;용Na2 S2 O3 적정법측정ACT청제H2 O2 적능력. ACT총당질량분수위(26 ±1.3) mg·mL-1,총다당질량분수위(12.5 ±0.8) mg·mL-1,총황동질량분수위(121 ±8.5) μg ·mL-1. ACT가일정정도상청제O2 ·-、·OH화H2 O2. ACT구유온화적항양화작용,종이유지궤체양화여항양화평형,저시ACT촉진건강적잠재궤제.
Astragalus membranaceus/Codonopsis pilosula Qi-invigorating herbal tea ( ACT) was prepared from Astragalus membranaceus and Codonopsis pilosula according to traditional Chinese medicine principle. Mitochondrial reactive oxygen species scavenging activity of ACT and the underlying health - promoting mechanism were investigated. Total flavonoids, total carbohy-drates and total polysaccharides, which are the functional factors of ACT, were determined by AlCl3 colorimetry, and sulfuric acid and phenol method respectively. The scavenging activities of ACT on superoxide anion ( O2 ·-) and hydroxyl radical ( ·OH) , which were produced by re-duced nicotinamide adenine dinucleotide ( NADH )/N - Methylphenazonium methyl sulfate ( PMS) and hydrogen peroxide ( H2 O2 )/Fe2+ system respectively, were measured by NBT re-duction and Fenton reaction colorimetry. The Na2 S2 O3 titration method was used to measure the scavenging activities of ACT on H2 O2 . The contents of total carbohydrates, total polysaccharides and total flavonoids of ACT were 26 ± 1. 3 mg/mL, 12. 5 ± 0. 8 mg/mL and 121 ± 8. 5 μg/mL, respectively. ACT could scavenge O2·-, ·OH and H2O2. According to the research, ACT has mild antioxidation, therefore, the balance of oxidation and antioxidant ( redox balance) was maintained, and this maybe the underlying mechanism of the health benefits for ACT.