CAJ | 학술논문

目的：采用SAX-HPLC快速鉴别不同种属来源的粗品肝素钠。方法通过肝素酶酶解不同种属来源的粗品肝素钠，以其酶解后的二糖组份为考察对象，通过SAX HPLC法比较二糖的相对组成，鉴定种属来源。同时在猪来源肝素中分别添加不同比例（5%，10%，15%，20%，25%）的牛来源肝素或羊来源肝素，经肝素酶酶解后，通过SAX HPLC法鉴别在猪来源肝素中是否能快速检出不同种属来源的其他肝素。结果对于不同种属来源的粗品肝素钠，它们的二糖组成具有显著差异；若猪来源肝素混入5%羊来源肝素，即可通过其二糖组成判断。结论此法操作简便，成本较低，可用于不同种属来源的粗品肝素钠的定性检测。
목적：채용SAX-HPLC쾌속감별불동충속래원적조품간소납。방법통과간소매매해불동충속래원적조품간소납，이기매해후적이당조빈위고찰대상，통과SAX HPLC법비교이당적상대조성，감정충속래원。동시재저래원간소중분별첨가불동비례（5%，10%，15%，20%，25%）적우래원간소혹양래원간소，경간소매매해후，통과SAX HPLC법감별재저래원간소중시부능쾌속검출불동충속래원적기타간소。결과대우불동충속래원적조품간소납，타문적이당조성구유현저차이；약저래원간소혼입5%양래원간소，즉가통과기이당조성판단。결론차법조작간편，성본교저，가용우불동충속래원적조품간소납적정성검측。
Objective To identify the origin of species of crude heparin sodium by SAX-HPLC.Methods With the digested disaccharide components as the study object which was got through heparinase hydrolysis of crude heparin sodium, SAX-HPLC was used to identify the origin of species by the comparison of disaccharide relative composition. Meanwhile, bovine-or ovine-sourced heparin was added to the porcine crude heparin at different proportions (5 %,10 %,15 %,20 %,25 %). After the digestion by heparinase, it was confirmed whether heparin from foreign species could be detected quickly within porcine crude heparin by SAX-HPLC.ResultsThere were significant differences on disaccharide composition among crude heparin from different species. If porcine crude heparin was mixed with 5 % ovine crude heparin, it could be detected by the disaccharide composition.Conclusion This method is simple and low-cost, and can be used for the qualitative determination of crude heparin sodium from different species.