中华医学杂志
中華醫學雜誌
중화의학잡지
National Medical Journal of China
2015年
37期
3061-3065
,共5页
骨%人工器官%内皮生长因子%基因,调节%生物医学工程
骨%人工器官%內皮生長因子%基因,調節%生物醫學工程
골%인공기관%내피생장인자%기인,조절%생물의학공정
Bone%Artificial organs%Endothelial groeth factors%Genes,regular%Biomedical engineering
目的 观察血管内皮生长因子(VEGF)基因、骨髓间充质干细胞(BMSCs)和nano-羟基磷灰石(HA)/聚乳酸羟基乙酸(PLGA)支架材料构建可分泌促进成血管活性细胞因子能力工程骨的相容性.方法 以BMSCs为靶细胞进行VEGF基因转染,观察转染及基因的表达情况.将BMSCs及转染细胞与nano-羟基磷灰石(HA)/聚乳酸羟基乙酸(PLGA)支架材料复合,观察细胞与支架材料的复合情况.结果 转染结果显示空白质粒转染率为39.1％、VEGF组为40.1％.接种5d后,细胞的黏附在支架孔壁上,两组细胞黏附数量差异无统计学意义(P＞0.05).结论 BMSCs细胞转染VEGF基因后,能够良好存活并与nano-HA/PLGA支架材料复合,高效率表达VEGF,为保证工程骨具有良好生物活性提供必要条件.
目的 觀察血管內皮生長因子(VEGF)基因、骨髓間充質榦細胞(BMSCs)和nano-羥基燐灰石(HA)/聚乳痠羥基乙痠(PLGA)支架材料構建可分泌促進成血管活性細胞因子能力工程骨的相容性.方法 以BMSCs為靶細胞進行VEGF基因轉染,觀察轉染及基因的錶達情況.將BMSCs及轉染細胞與nano-羥基燐灰石(HA)/聚乳痠羥基乙痠(PLGA)支架材料複閤,觀察細胞與支架材料的複閤情況.結果 轉染結果顯示空白質粒轉染率為39.1％、VEGF組為40.1％.接種5d後,細胞的黏附在支架孔壁上,兩組細胞黏附數量差異無統計學意義(P＞0.05).結論 BMSCs細胞轉染VEGF基因後,能夠良好存活併與nano-HA/PLGA支架材料複閤,高效率錶達VEGF,為保證工程骨具有良好生物活性提供必要條件.
목적 관찰혈관내피생장인자(VEGF)기인、골수간충질간세포(BMSCs)화nano-간기린회석(HA)/취유산간기을산(PLGA)지가재료구건가분비촉진성혈관활성세포인자능력공정골적상용성.방법 이BMSCs위파세포진행VEGF기인전염,관찰전염급기인적표체정황.장BMSCs급전염세포여nano-간기린회석(HA)/취유산간기을산(PLGA)지가재료복합,관찰세포여지가재료적복합정황.결과 전염결과현시공백질립전염솔위39.1％、VEGF조위40.1％.접충5d후,세포적점부재지가공벽상,량조세포점부수량차이무통계학의의(P＞0.05).결론 BMSCs세포전염VEGF기인후,능구량호존활병여nano-HA/PLGA지가재료복합,고효솔표체VEGF,위보증공정골구유량호생물활성제공필요조건.
Objective To explorec Histocompatibility of nano-hydroxyapatite/poly-co-glycolic acid tissue engineering bone modified by mesenchymal stem cells with vascular endothelial frowth factor transinfected.Methods Rat bone marrow mesenchymal stem cells (BMSCs) was separated,using BMSCs as target cells,and then vascular endothelial growth factor (VEGF) gene was transfected.Composite bone marrow mesenchymal stem cells and cells transfected with nano-hydroxyapatite (HA)/polylactic-co-glycolic acid(PLGA).The composition of cell and scaffold was observed.Results The blank plasmid transfection was 39.1％,40.1％ in VEGF group.The cell adhesion and growth was found on the scaffold pore wall after 5 days,and the number of adherent cells in the nano-HA/PLGA composite scaffold material basically had no significant difference in both.Conclusion Although the nano-HA/PLGA scaffold material is still not fully meet the requirements of the matrix material for bone tissue engineering,but good biocompatibility,structure is its rich microporous satisfaction in material mechanics,toughening,enhanced obviously.Composition scaffold with BMSCs transfected by VEGF plasmid,the ability of angiogenesis is promoted.
