医学综述
醫學綜述
의학종술
Medical Recapitulate
2015年
18期
3412-3414
,共3页
刘志刚%熊敏%唐冰%李锋%余化龙%曾云%陈洁%何宁%王志勇%韩珩
劉誌剛%熊敏%唐冰%李鋒%餘化龍%曾雲%陳潔%何寧%王誌勇%韓珩
류지강%웅민%당빙%리봉%여화룡%증운%진길%하저%왕지용%한형
人骨髓间充质干细胞%成骨细胞%定向分化%地塞米松%碱性磷酸酶
人骨髓間充質榦細胞%成骨細胞%定嚮分化%地塞米鬆%堿性燐痠酶
인골수간충질간세포%성골세포%정향분화%지새미송%감성린산매
Human mesenchymal stem cells%Osteoblasts%Directed differentiation%Dexamethasone%Alkaline phosphatase
目的：探讨人骨髓间充质干细胞在体外向成骨细胞定向分化情况。方法取引产胎儿的人骨髓间充质干细胞进行原代细胞培养，诱导培养向成骨细胞分化，诱导后进行形态学观察，碱性磷酸酶（ ALP）染色与生长曲线测定。当人骨髓间充质干细胞培养生长密度为80.0％左右时分为转染组与对照组，对照组不进行转染，转染组常规进行转染。结果原代细胞生长2h开始贴壁，培养4d左右胞体逐渐变得粗大。经成骨诱导后细胞形态由长梭形逐渐变为多边形，形成细胞结节甚至团块状。转染组的胎儿骨髓间质干细胞和未转染对照组的生长状况对比差异无统计学意义（ P＞0.05），转染细胞与未转染细胞倍增时间分别为（23±4） h和（25±4） h。转染组在转染后第3、6、9、12日的ALP活性均明显高于对照组[（0.12±0.02）％比（0.10±0.02）％，（0.16±0.02）％比（0.12±0.02）％，（0.20±0.03）％比（0.13±0.02）％，（0.23±0.04）％比（0.14±0.02）％]，差异有统计学意义（ P＜0.01）。结论胎儿骨髓间充质干细胞在成骨细胞分化转染的诱导下可促进向成骨细胞进行定向分化，为胎儿间质干细胞分化调控和药物作用机制研究奠定基础。
目的：探討人骨髓間充質榦細胞在體外嚮成骨細胞定嚮分化情況。方法取引產胎兒的人骨髓間充質榦細胞進行原代細胞培養，誘導培養嚮成骨細胞分化，誘導後進行形態學觀察，堿性燐痠酶（ ALP）染色與生長麯線測定。噹人骨髓間充質榦細胞培養生長密度為80.0％左右時分為轉染組與對照組，對照組不進行轉染，轉染組常規進行轉染。結果原代細胞生長2h開始貼壁，培養4d左右胞體逐漸變得粗大。經成骨誘導後細胞形態由長梭形逐漸變為多邊形，形成細胞結節甚至糰塊狀。轉染組的胎兒骨髓間質榦細胞和未轉染對照組的生長狀況對比差異無統計學意義（ P＞0.05），轉染細胞與未轉染細胞倍增時間分彆為（23±4） h和（25±4） h。轉染組在轉染後第3、6、9、12日的ALP活性均明顯高于對照組[（0.12±0.02）％比（0.10±0.02）％，（0.16±0.02）％比（0.12±0.02）％，（0.20±0.03）％比（0.13±0.02）％，（0.23±0.04）％比（0.14±0.02）％]，差異有統計學意義（ P＜0.01）。結論胎兒骨髓間充質榦細胞在成骨細胞分化轉染的誘導下可促進嚮成骨細胞進行定嚮分化，為胎兒間質榦細胞分化調控和藥物作用機製研究奠定基礎。
목적：탐토인골수간충질간세포재체외향성골세포정향분화정황。방법취인산태인적인골수간충질간세포진행원대세포배양，유도배양향성골세포분화，유도후진행형태학관찰，감성린산매（ ALP）염색여생장곡선측정。당인골수간충질간세포배양생장밀도위80.0％좌우시분위전염조여대조조，대조조불진행전염，전염조상규진행전염。결과원대세포생장2h개시첩벽，배양4d좌우포체축점변득조대。경성골유도후세포형태유장사형축점변위다변형，형성세포결절심지단괴상。전염조적태인골수간질간세포화미전염대조조적생장상황대비차이무통계학의의（ P＞0.05），전염세포여미전염세포배증시간분별위（23±4） h화（25±4） h。전염조재전염후제3、6、9、12일적ALP활성균명현고우대조조[（0.12±0.02）％비（0.10±0.02）％，（0.16±0.02）％비（0.12±0.02）％，（0.20±0.03）％비（0.13±0.02）％，（0.23±0.04）％비（0.14±0.02）％]，차이유통계학의의（ P＜0.01）。결론태인골수간충질간세포재성골세포분화전염적유도하가촉진향성골세포진행정향분화，위태인간질간세포분화조공화약물작용궤제연구전정기출。
Objective To discuss human bone marrow mesenchymal stem cells differentiation into osteo-blasts in vitro.Methods Human bone marrow mesenchymal stem cells of induced labor fetus were taken for primary culture to induce osteoblast differentiation,the morphology was observed,the alkaline phosphatase (ALP) staining and the growth curve were determined.When human bone marrow mesenchymal stem cell culture growth density was about 80.0%,they were divided into two groups:transfection group and control group,the control group was not transfected, the transfection group was givenconventional transfection. Results The primary cell were adherent growth after 2 h and showed stretch fusiform adherent cells after cultured for 4 days.The cell body gradually became thick and showed elongated protrusions.After inducing, the cells showed morphology.The cells growth conditions between the transfected fetal bone marrow mesen-chymal stem cells and the untransfected cells showed no significant difference(P>0.05).The doubling time of the transfected cells and non-transfected cells were (23 ±4) h and (25 ±4) h respectively.The ALP activity of the transfection group was significantly higher at different times [(0.12 ±0.02)% vs ( 0.10 ± 0.02)%,(0.16 ±0.02)% vs (0.12 ±0.02)%,(0.20 ±0.03)% vs (0.13 ±0.02)%,(0.23 ± 0.04)% vs (0.14 ±0.02)%] (P <0.01).Conclusion Human bone marrow mesenchymal stem cells directly differentiated into osteoblasts in vitro under induction ,which can be the basis for further fetal intersti-tial stem cell differentiation regulation and study on the drug action mechanisms .
