CAJ | 학술논문

目的：探讨小儿喘息性疾病血清总免疫球蛋白E (tIgE)和特异性免疫球蛋白E (sIgE)变化水平。方法选取2013年9月至2015年6月我院收治256例喘息性疾病患儿作为研究组，选取同期我院体检中心体检健康儿童100例作为健康组。采用酶联免疫吸附法检测tIgE水平，欧蒙印迹法检测sIgE水平。比较两组儿童tIgE、 sIgE水平，并分析喘息性疾病患儿中血清tIgE、sIgE的相关性。结果研究组患儿tIgE、 sIgE水平均显著高于健康组，差异有统计学意义(P＜0.05)。研究组患儿tIgE升高179例，正常77例；升高组sIgE阳性率为62.57%(112/179)，明显高于正常组的7.79%(6/77)，差异有统计学意义(P＜0.05)。结论喘息疾病患儿血清tIgE、 sIgE水平明显升高， tIgE升高患儿中sIgE也呈明显升高，两者联合检测可提高小儿哮喘检出率。
목적：탐토소인천식성질병혈청총면역구단백E (tIgE)화특이성면역구단백E (sIgE)변화수평。방법선취2013년9월지2015년6월아원수치256례천식성질병환인작위연구조，선취동기아원체검중심체검건강인동100례작위건강조。채용매련면역흡부법검측tIgE수평，구몽인적법검측sIgE수평。비교량조인동tIgE、 sIgE수평，병분석천식성질병환인중혈청tIgE、sIgE적상관성。결과연구조환인tIgE、 sIgE수평균현저고우건강조，차이유통계학의의(P＜0.05)。연구조환인tIgE승고179례，정상77례；승고조sIgE양성솔위62.57%(112/179)，명현고우정상조적7.79%(6/77)，차이유통계학의의(P＜0.05)。결론천식질병환인혈청tIgE、 sIgE수평명현승고， tIgE승고환인중sIgE야정명현승고，량자연합검측가제고소인효천검출솔。
Objective To investigate the changes of serum total immunoglobulin E (tIgE) and specific immunoglobulin E (sIgE) in pediatric asthmatic disease. Methods 256 children with asthmatic disease treated in our hospital from September 2013 to June 2015 were set as study group, 100 healthy children receiving physical examination in our hospital in same period were set as healthy group. The tIgE level was detected by ELISA, the sIgE level was detected by EUROLine. The levels of tIgE and sIgE were compared, the correlation between tIgE and sIgE in children with asthmatic disease was analyzed. Results The level of tIgE and sIgE in study group were higher than those in healthy group, with statistical difference (P<0.05). Among study group, the tIgE of 179 cases increased, and 77 cases maintained a normal level;the positive rate of sIgE in raise group was 62.57%(112/179), higher than 7.79%(6/77) in normal group (P<0.05). Conclusions The level of tIgE and sIgE in children with asthmatic disease increases significantly, and the level of sIgE in children with high tIgE also increases significantly. The joint detection can improve the detection rate of asthma in children.