刑事技术
刑事技術
형사기술
Forensic Science and Technology
2015年
5期
359-363
,共5页
丰蕾%杨帆%李彩霞%徐珍%凃政%李万水%胡兰
豐蕾%楊帆%李綵霞%徐珍%凃政%李萬水%鬍蘭
봉뢰%양범%리채하%서진%도정%리만수%호란
法医遗传学%单细胞分离检验技术%微量混合样本%单细胞测序%二代测序
法醫遺傳學%單細胞分離檢驗技術%微量混閤樣本%單細胞測序%二代測序
법의유전학%단세포분리검험기술%미량혼합양본%단세포측서%이대측서
forensic genetics%single cell separation technique%trace and mixture sample%single-cell sequencing%next generation sequencing
法医单细胞分离检验技术是微量混合 DNA 样本检验最有效的方法之一。常规的单细胞分离检验技术包括单细胞分离、单细胞裂解、PCR 扩增、数据分析。本文将详细介绍单细胞分离检验技术原理和应用进展,同时展望了单细胞测序在法医 DNA 的应用前景。近5年来,单细胞分离、全基因组扩增和二代测序技术的结合而产生的单细胞测序技术,将为法医遗传学检测提供一种全新的技术和思路,尤其为微量混合生物样品检验提供全新的技术手段。
法醫單細胞分離檢驗技術是微量混閤 DNA 樣本檢驗最有效的方法之一。常規的單細胞分離檢驗技術包括單細胞分離、單細胞裂解、PCR 擴增、數據分析。本文將詳細介紹單細胞分離檢驗技術原理和應用進展,同時展望瞭單細胞測序在法醫 DNA 的應用前景。近5年來,單細胞分離、全基因組擴增和二代測序技術的結閤而產生的單細胞測序技術,將為法醫遺傳學檢測提供一種全新的技術和思路,尤其為微量混閤生物樣品檢驗提供全新的技術手段。
법의단세포분리검험기술시미량혼합 DNA 양본검험최유효적방법지일。상규적단세포분리검험기술포괄단세포분리、단세포렬해、PCR 확증、수거분석。본문장상세개소단세포분리검험기술원리화응용진전,동시전망료단세포측서재법의 DNA 적응용전경。근5년래,단세포분리、전기인조확증화이대측서기술적결합이산생적단세포측서기술,장위법의유전학검측제공일충전신적기술화사로,우기위미량혼합생물양품검험제공전신적기술수단。
Forensic single cell separation testing technology is one of the most effective ways for trace and mixture DNA sample testing. Traditional single cell separation testing technology includes single cell isolation and lysis, PCR amplification and data analysis. This article details the principle of traditional single-cell separation and its application in case testing, meanwhile places the focus on prospect of single-cell sequencing (SCS) technology into forensic application. The research on SCS has shown tremendous growth over the past five years, and impacted many diverse areas of biological research. However, the study in forensic genetics is as yet waiting for developing. SCS is a combination of the single cell isolation, similar to the traditional single cell separation, with whole-genome amplification and next generation sequencing. In this review, the whole genome amplification and next generation sequencing (NGS) were summarized together with the advantages and disadvantages of the current technologies analyzed. Until now, there are some commercial kits of whole-genome amplification for biological research and ones of next generation sequencing for forensic application. Although the SCS technology is still relatively new, it has been being developed rapidly and will provide new means for forensic testing, especially for trace and mixture sample testing. The grounds root at the quantity-enormous parallel sequencing of NGS that is relied with SCS. This implementation can make one single sample tested hundreds of times so that such repetitious verification will definitely produce more complete and accurate information. Besides, next generation sequencing can bring the minor component in mixed sample into its STR types, even leading the mixture containing substance of three or more persons to evidential results, therefore exposing various genetic markers like STR, SNP from different individuals in just one single run of detection. Additionally, the real-time single molecule’s sequencing by the third generation sequencing will set a progressive aspiration for forensic genetics with the direct reading DNA sequence.