中国园艺文摘
中國園藝文摘
중국완예문적
ZHONGGUO YUANYI WENZHAI
2015年
9期
26-27,29
,共3页
张颖%潘丹丹%顾利利%唐晓倩
張穎%潘丹丹%顧利利%唐曉倩
장영%반단단%고리리%당효천
白花玫瑰%组织培养%芽诱导%增殖培养%生根培养
白花玫瑰%組織培養%芽誘導%增殖培養%生根培養
백화매괴%조직배양%아유도%증식배양%생근배양
White Rose%tissue culture%bud induction%proliferation culture%rooting culture
以白花玫瑰幼嫩茎段为外植体，对其离体培养途径进行研究，建立白花玫瑰的组织培养体系。结果表明：芽诱导培养基配方为：MS+6-BA 2.5 mg/L+NAA 0.3 mg/L+蔗糖30 g/L+琼脂6 g/L；增殖培养基配方为：MS+6-BA 3.0 mg/L+NAA 0.2 mg/L+蔗糖30 g/L+琼脂6 g/L；生根培养基配方为1/2 MS+NAA 0.2 ml/L+蔗糖30 g/L+琼脂6 g/L。
以白花玫瑰幼嫩莖段為外植體，對其離體培養途徑進行研究，建立白花玫瑰的組織培養體繫。結果錶明：芽誘導培養基配方為：MS+6-BA 2.5 mg/L+NAA 0.3 mg/L+蔗糖30 g/L+瓊脂6 g/L；增殖培養基配方為：MS+6-BA 3.0 mg/L+NAA 0.2 mg/L+蔗糖30 g/L+瓊脂6 g/L；生根培養基配方為1/2 MS+NAA 0.2 ml/L+蔗糖30 g/L+瓊脂6 g/L。
이백화매괴유눈경단위외식체，대기리체배양도경진행연구，건립백화매괴적조직배양체계。결과표명：아유도배양기배방위：MS+6-BA 2.5 mg/L+NAA 0.3 mg/L+자당30 g/L+경지6 g/L；증식배양기배방위：MS+6-BA 3.0 mg/L+NAA 0.2 mg/L+자당30 g/L+경지6 g/L；생근배양기배방위1/2 MS+NAA 0.2 ml/L+자당30 g/L+경지6 g/L。
The tissue culture system of White Rose was studied by using tender stem segmentsas explant.The results show that the bud inducing culturemedium formula was MS+2.5 mg/L 6-BA+0.3 mg/L NAA+30 g/L sucrose+6 g/L agar; The proliferation cultureformula was MS+3.0 mg/L 6-BA+0.2 mg/L NAA+30 g/L sucrose+6 g/L agar; The rooting cultural medium formula was 1/2 MS+0.2 mg/L NAA+30 g/L sucrose+6 g/L agar.
