CAJ | 학술논문

Objective:To explore the effect of quercetin on the epithelial-mesenchymal transition of human gastric carcinoma BGC-803 cells induced by TGFβ-1 and its mechanism.Methods:Gastric carcinoma BGC-803 cells were divided into three groups, i.e. normal control group, TGFβ-1, and TGFβ-1 + quercetin group. After 12, 24, 48, and 72 h, MTT was used to detect the cell proliferation capability, scratch repair experiment was used to detect the cell migration ability, and Western blot was utilized for determining the expressions of epithelial-mesenchymal transition factor, E-cadherin, N-cadherin and Vimentin. Meanwhile, the activity of PI3K/AKT signal transduction pathway was evaluated.Results:When compared with the TGFβ-1 group, quercetin reduced the proliferation capability and migration ability of human gastric carcinoma BGC-803 cells. In the TGFβ-1 + quercetin group, E-cadherin expression level was up regulated, N-cadherin, Vimentin, AKT, and p-AKT expression levels were down regulated, and the activity of PI3K/AKT signal transduction pathway was reduced. Conclusions:Quercetin can inhibit the EMT process of human gastric cancer cell lines induced by TGFβ-1. The pathogenetic mechanism is probably associated with the regulation of PI3K/AKT signal transduction pathway activity.