中华实验和临床病毒学杂志
中華實驗和臨床病毒學雜誌
중화실험화림상병독학잡지
Chinese Journal of Experimental and Clinical Virology
2015年
5期
425-429
,共5页
苏祥%刘志荣%刘建伟%刘长梅%温红玲%宋艳艳%宋修光%于学杰%赵丽
囌祥%劉誌榮%劉建偉%劉長梅%溫紅玲%宋豔豔%宋脩光%于學傑%趙麗
소상%류지영%류건위%류장매%온홍령%송염염%송수광%우학걸%조려
脑炎病毒,日本%西尼罗病毒%酶联免疫吸附测定%抗体效价
腦炎病毒,日本%西尼囉病毒%酶聯免疫吸附測定%抗體效價
뇌염병독,일본%서니라병독%매련면역흡부측정%항체효개
Encephalitis virus Japanese%West Nile virus%Enzyme-linked immunosorbent assay%antibody titer
目的 研究山东省是否存在西尼罗病毒(WNV)感染.方法 收集山东省部分地区2013年242例临床诊断为流行性乙型脑炎(JE)患者的样本共计272份(急性期血清和脑脊液214份、恢复期血清58份).利用逆转录PCR检测急性期样本中乙型脑炎病毒(JEV)和WNV的RNA,并对阳性样本进行病毒分离,用酶联免疫吸附试验(ELISA)检测样本中JEV和WNV的IgM和IgG抗体.对JEV-IgM和WNV-IgM、JEV-IgG和WNV-IgG抗体出现交叉反应的样本进一步检测其抗体效价.将下列两种结果视为WNV感染可疑病例:①两种病毒抗体检测均阳性的样本:抗体效价WNV-IgM/JEV-IgM≥4或WNV-IgG/JEV-IgG≥4者.②WNV-IgM(+)、JEV-IgM(-)或WNV-IgG(+)、JEV-IgG(-)的样本.结果 214份急性期样本中有4份JEV RNA的RT-PCR结果(+),未扩增出WNVRNA,未分离到病毒.急性期样本中,JEV-IgM阳性率为99.07%(212/214),WNV-IgM阳性率为35.05% (75/214),交叉反应率为34.58% (74/214);恢复期样本中,JEV-IgG阳性率为55.17% (32/58),WNV-IgG阳性率为46.55% (27/58),交叉反应率为为31.03% (18/58);WNV感染可疑病例15例.结论 山东部分地区2013年JE患者中可能存在WNV感染病例,但本次研究的结果不能完全支持山东省存在WNV流行的情况,提示在该地区加强JE防控的同时应增加WNV的监测.
目的 研究山東省是否存在西尼囉病毒(WNV)感染.方法 收集山東省部分地區2013年242例臨床診斷為流行性乙型腦炎(JE)患者的樣本共計272份(急性期血清和腦脊液214份、恢複期血清58份).利用逆轉錄PCR檢測急性期樣本中乙型腦炎病毒(JEV)和WNV的RNA,併對暘性樣本進行病毒分離,用酶聯免疫吸附試驗(ELISA)檢測樣本中JEV和WNV的IgM和IgG抗體.對JEV-IgM和WNV-IgM、JEV-IgG和WNV-IgG抗體齣現交扠反應的樣本進一步檢測其抗體效價.將下列兩種結果視為WNV感染可疑病例:①兩種病毒抗體檢測均暘性的樣本:抗體效價WNV-IgM/JEV-IgM≥4或WNV-IgG/JEV-IgG≥4者.②WNV-IgM(+)、JEV-IgM(-)或WNV-IgG(+)、JEV-IgG(-)的樣本.結果 214份急性期樣本中有4份JEV RNA的RT-PCR結果(+),未擴增齣WNVRNA,未分離到病毒.急性期樣本中,JEV-IgM暘性率為99.07%(212/214),WNV-IgM暘性率為35.05% (75/214),交扠反應率為34.58% (74/214);恢複期樣本中,JEV-IgG暘性率為55.17% (32/58),WNV-IgG暘性率為46.55% (27/58),交扠反應率為為31.03% (18/58);WNV感染可疑病例15例.結論 山東部分地區2013年JE患者中可能存在WNV感染病例,但本次研究的結果不能完全支持山東省存在WNV流行的情況,提示在該地區加彊JE防控的同時應增加WNV的鑑測.
목적 연구산동성시부존재서니라병독(WNV)감염.방법 수집산동성부분지구2013년242례림상진단위류행성을형뇌염(JE)환자적양본공계272빈(급성기혈청화뇌척액214빈、회복기혈청58빈).이용역전록PCR검측급성기양본중을형뇌염병독(JEV)화WNV적RNA,병대양성양본진행병독분리,용매련면역흡부시험(ELISA)검측양본중JEV화WNV적IgM화IgG항체.대JEV-IgM화WNV-IgM、JEV-IgG화WNV-IgG항체출현교차반응적양본진일보검측기항체효개.장하렬량충결과시위WNV감염가의병례:①량충병독항체검측균양성적양본:항체효개WNV-IgM/JEV-IgM≥4혹WNV-IgG/JEV-IgG≥4자.②WNV-IgM(+)、JEV-IgM(-)혹WNV-IgG(+)、JEV-IgG(-)적양본.결과 214빈급성기양본중유4빈JEV RNA적RT-PCR결과(+),미확증출WNVRNA,미분리도병독.급성기양본중,JEV-IgM양성솔위99.07%(212/214),WNV-IgM양성솔위35.05% (75/214),교차반응솔위34.58% (74/214);회복기양본중,JEV-IgG양성솔위55.17% (32/58),WNV-IgG양성솔위46.55% (27/58),교차반응솔위위31.03% (18/58);WNV감염가의병례15례.결론 산동부분지구2013년JE환자중가능존재WNV감염병례,단본차연구적결과불능완전지지산동성존재WNV류행적정황,제시재해지구가강JE방공적동시응증가WNV적감측.
Objective To determine whether human infection with West Nile Virus in Shandong province.Methods 272 clinical samples (214 sera and cerebrospinal fluids of acute phase,58 sera of convalescent phase) from 242 patients who were clinically diagnosed as Japanese encephalitis (JE) in parts of Shandong province were collected.RNAs of Japanese encephalitis virus (JEV) and WNV were detected by RT-PCR from acute phase samples,and positive samples were used for virus isolation,IgM and IgG antibodies to JEV and WNV of samples were detected by Enzyme-linked immunosorbent assay (ELISA).JEV-IgM and WNV-IgM,JEV-IgG and WNV-IgG cross-reacted samples were further detected antibody titers.The following two results were considered as potential WNV infected cases:① Both antibody positive samples:For antibody titers,WNV-IgM/JEV-IgM ≥4 or WNV-IgG/JEV-IgG≥4.②WNV-IgM (+) and JEV-IgM (-),or WNV-IgG (+) and JEV-IgG (-) samples.Results 4 of 214 acute phase samples were positive to JEV RNA,but all samples were negative to WNV RNA by RT-PCR.No virus was isolated from the samples.In acute samples,JEV-IgM positive rate was 99.07% (212/214),WNV-IgM positive rate was 35.05% (75/214),cross-reaction rate was 34.58% (74/214).In convalescent samples,JEV-IgG positive rate was 55.17% (32/58),WNV-IgG positive rate was 46.55% (32/58),cross-reaction rate was 31.03% (18/58).15 patients were considered as potential WNV infection.Conclusions Our study indicated there may be some cases infected with WNV from JE patients in Shandong Province of China,2013,but the results can not fully support the epidemic of WNV in this area,surveillance of WNV should be strengthened meanwhile JE was prevented and controlled.