CAJ | 학술논문

目的探讨上海地区急性呼吸道感染(A RTI)人群中小RNA病毒感染的流行规律及临床特征.方法采用套式多重RT-PCR方法,同时检测鼻咽拭子标本小RNA病毒和其他8种常见呼吸道病毒.对其中小RNA病毒阳性标本进行基因分型,并进一步分析其流行病学和临床特征.结果 2282份鼻咽拭子标本中检出小RNA病毒阳性185例(8.1％),其中鼻病毒(HRV)为151(81.6％)例,肠道病毒(EV)为34(18.4％)例.HRV-A(61.6％)是HRV的主要亚型.HRV在0～4岁组及65岁以上组中检出率高于FluA、RSV等常见呼吸道病毒.HRV和EV在不同季节均有检出,其中HRV在秋冬季达到高峰.HRV引起的URTI和LRTI的比例分别为62.3％ (94/151)、37.8％ (57/151),而EV感染病例均为URTI.HRV感染阳性的LRTI病例中亚型与LRTI的发生无显著相关性(P＞0.05).结论本研究表明,2009-2011年小RNA病毒成为上海地区急性呼吸道感染谱的重要组成部分,其中HRV(HRV-A)为主要病原.HRV是0～4岁组及65岁以上组中最常见的呼吸道病毒,并以秋冬两季为高发季节.HRV亚型与临床重症并无相关性.
목적 탐토상해지구급성호흡도감염(A RTI)인군중소RNA병독감염적류행규률급림상특정.방법 채용투식다중RT-PCR방법,동시검측비인식자표본소RNA병독화기타8충상견호흡도병독.대기중소RNA병독양성표본진행기인분형,병진일보분석기류행병학화림상특정.결과 2282빈비인식자표본중검출소RNA병독양성185례(8.1％),기중비병독(HRV)위151(81.6％)례,장도병독(EV)위34(18.4％)례.HRV-A(61.6％)시HRV적주요아형.HRV재0～4세조급65세이상조중검출솔고우FluA、RSV등상견호흡도병독.HRV화EV재불동계절균유검출,기중HRV재추동계체도고봉.HRV인기적URTI화LRTI적비례분별위62.3％ (94/151)、37.8％ (57/151),이EV감염병례균위URTI.HRV감염양성적LRTI병례중아형여LRTI적발생무현저상관성(P＞0.05).결론 본연구표명,2009-2011년소RNA병독성위상해지구급성호흡도감염보적중요조성부분,기중HRV(HRV-A)위주요병원.HRV시0～4세조급65세이상조중최상견적호흡도병독,병이추동량계위고발계절.HRV아형여림상중증병무상관성.
Objective The present paper aims to investigate the epidemiological and clinical characteristics of patients with ARTIs infections in a large cohort.Methods Nasopharyngeal swabs of patients with ARTIs from Shanghai during May 2009 to April 2011.The nested multiplex reverse transcriptase-polymerase chain reaction (RT-PCR) was used to detect picornavirus and other 8 respiratory viruses.All picornavirus samples were subtyped by nested RT-PCR that targeted to VP4/2 or VP1 region.Results Among the 2282 nasopharyngeal swabs,the detection rate of picornavirus was 8.1％ and they were consisted with HRV (151,81.6％) and EV (34,18.4％).HRV-A (93/151,61.6％) was the most common species of HRV.HRV and RSV were most common in patients younger than 5 years-old,and HRV and Flu A were most common in patients who were at least 65 years-old.HRV-A was the most common HRV species of all age groups.A total of 57 HRV-infected patients (37.8％) presented with lower respiratory tract infections (LRTIs),including HRV-A (61.6％),HRV-B (14.1％) and HRV-C (24.5％).There was no correlation between the HRV species and LRTI.Conclusions Our study indicated that picornavirus was the important pathogen in ARTI patients in Shanghai from May 2009 to April 2011,espacially HRV (HRV-A).HRV was the most common virus in the younger than 5 years-old and the at least 65 years-old group,the peak season was in autum/winter.No specific HRV subtype correlated with LRTI.