CAJ | 학술논문

目的了解淮安市某中学甲型H1N1流感暴发的分子流行病学特征,为甲型H1N1流感疫情的控制提供依据.方法采用现场流行病学方法,对甲型H1N1流感疫情进行调查分析;对病例标本进行病毒分离;采用巢式PCR扩增甲型H1N1流感病毒HA1基因的核苷酸序列,利用MEGA5.0分析软件,进行同源性分析和构建系统进化树.结果疫情持续11天共确诊甲型H1N1流感样病例51例,罹患率为2.49％ (51/2051).序列进化分析表明,本次疫情的病毒基因与2014年和2013年甲型H1N1流感病毒HA1基因核苷酸序列的同源性为分别为99.6％～ 99.9％和98.5％～98.8％,与2011年之前分离的核苷酸同源性为98.4％.结论此次疫情流行程度较低,及时隔离病人及密切接触者在控制疫情中起到了重要作用;病毒基因变异程度不高,加强健康教育和疫苗接种有助于预防流感.
목적 료해회안시모중학갑형H1N1류감폭발적분자류행병학특정,위갑형H1N1류감역정적공제제공의거.방법 채용현장류행병학방법,대갑형H1N1류감역정진행조사분석;대병례표본진행병독분리;채용소식PCR확증갑형H1N1류감병독HA1기인적핵감산서렬,이용MEGA5.0분석연건,진행동원성분석화구건계통진화수.결과 역정지속11천공학진갑형H1N1류감양병례51례,리환솔위2.49％ (51/2051).서렬진화분석표명,본차역정적병독기인여2014년화2013년갑형H1N1류감병독HA1기인핵감산서렬적동원성위분별위99.6％～ 99.9％화98.5％～98.8％,여2011년지전분리적핵감산동원성위98.4％.결론 차차역정류행정도교저,급시격리병인급밀절접촉자재공제역정중기도료중요작용;병독기인변이정도불고,가강건강교육화역묘접충유조우예방류감.
Objective To study the epidemiological characters of an influenza A/H1N1 outbreak in a middle school in Huaian city,and to provide scientific measures for prevention and control of influenza A/ H1N1.Method The outbreak of influenza A/H1N1 was investigated through the field epidemiology.The samples were used for virus isolation.Nucleotide sequences of H1N1 HA1 region were determined by RT-nested-PCR and sequencing.H1N1 genotypes were analyzed using bioinformatic programs by MEGA 5.0 software,and phylogenetic tree was constructed.Result 51 cases were reported during 11 days,the attack rate was 2.49％ (51/2051).The sequence analysis showed that the sequence homologous rates of H1N1 virus HA gene nucleotide were 99.6％-99.9％ with H1N1 genotype in 2014,98.5％-98.8％ in 2013,and lower than 98.4％ in 2011.Conclusion The popularity of this outbreak was low.Timely isolation of H1 N1 patients and close contacts played an important role in control the epidemics.Implementation of health propaganda and immunization of influenza A/H1N1 should be strengthened.