CAJ | 학술논문

目的：探讨不同剂量黄连素对慢性阻塞性肺疾病(COPD)模型大鼠气道炎症的影响。方法50只清洁级雄性Wistar大鼠随机分为生理盐水对照组、COPD模型组和低剂量(25 mg/kg)、中剂量(50 mg/kg)、高剂量(100 mg/kg)黄连素组，每组各10只。通过气管注射内毒素加熏香烟方法建立大鼠COPD模型。采集左肺支气管肺泡灌洗液，利用瑞氏-吉姆萨染色法行中性粒细胞(PMN)、肺泡巨噬细胞(AM)计数；ELISA法检测IL-8、IL-10、IL-17含量。结果 COPD模型组大鼠支气管肺泡灌洗液中的PMN、AM计数及IL-8、IL-17含量显著高于生理盐水对照组(P<0.01)；IL-10含量显著低于生理盐水对照组(P<0.01)。而不同剂量黄连素干预后PMN、AM计数，IL-8、IL-17水平有不同程度下降(P<0.01)，IL-10水平有不同程度升高(P<0.01)，且黄连素剂量越高，此效应越明显。结论不同剂量黄连素均可抑制COPD模型大鼠的气道炎症，其机制可能与黄连素下调炎症因子IL-8、IL-17水平，增强抗炎因子IL-10水平这一途径有关，且此效应与黄连素剂量呈正相关。
목적：탐토불동제량황련소대만성조새성폐질병(COPD)모형대서기도염증적영향。방법50지청길급웅성Wistar대서수궤분위생리염수대조조、COPD모형조화저제량(25 mg/kg)、중제량(50 mg/kg)、고제량(100 mg/kg)황련소조，매조각10지。통과기관주사내독소가훈향연방법건립대서COPD모형。채집좌폐지기관폐포관세액，이용서씨-길모살염색법행중성립세포(PMN)、폐포거서세포(AM)계수；ELISA법검측IL-8、IL-10、IL-17함량。결과 COPD모형조대서지기관폐포관세액중적PMN、AM계수급IL-8、IL-17함량현저고우생리염수대조조(P<0.01)；IL-10함량현저저우생리염수대조조(P<0.01)。이불동제량황련소간예후PMN、AM계수，IL-8、IL-17수평유불동정도하강(P<0.01)，IL-10수평유불동정도승고(P<0.01)，차황련소제량월고，차효응월명현。결론불동제량황련소균가억제COPD모형대서적기도염증，기궤제가능여황련소하조염증인자IL-8、IL-17수평，증강항염인자IL-10수평저일도경유관，차차효응여황련소제량정정상관。
Objective To investigate the effects of Berberine on the airway inflammation of rats with chronic obstructive pulmonary disease. Methods Fifty male and healthy Wistar rats were randomly divided into five groups, with 10 rats in each group, including NS control group, COPD group and 25, 50, 100 mg/kg Berberine group. Chronic obstructive pul-monary disease rats models were established by passive cigarette smoking plus intratracheal instillation of lipopolysac-charide. Bronchoalveolar lavage fluid (BALF) of left lung sample were collected. Polymorphonuclear (PMN) and alveolar macrophage (AM) counts in BALF were measured with Wright-Giemsa staining method. Interleukin-8 (IL-8), inter-leukin-10 (IL-10), interleukin-17 (IL-17) were detected with enzyme linked immunosorbent assay. Results The levels of PMN, AM counts and IL-8, IL-17 levels were significantly increased in BALF of COPD model group compared with saline control group (P<0.01), however, the level of IL-10 was just on the contrary (P< 0.01). After intervention with different doses of Berberine, compared with COPD model group, the levels of PMN, AM counts and IL-8, IL-17 levels were significantly decreased in BALF (P< 0.01), however, the level of IL-10 was increased to varying degrees (P<0.01), and the higher the dose of Berberine, this effects were more obvious. Conclusion Different doses of Berberine can inhibit the airway inflammation of COPD rat model. The mechanism is associated with Berberine lowered levels of inflammatory cytokine IL-8, IL-17 and enhance the level of anti-inflammatory cytokine IL-10, and this positive effect of Berberine is related to its dose.