CAJ | 학술논문

目的：探讨内皮祖细胞在噪声性耳聋耳蜗损伤中的变化。方法50只SD大鼠分为两组，其中空白对照组10只，实验组40只。空白对照组不做处理，实验组进行噪声暴露构建噪声性耳聋动物模型，并按照暴露时间分为4组，分别为暴露后即刻组和暴露后1 d组、暴露后7 d组、暴露后14 d组，每组各10只。分别检测各组动物的外周血内皮祖细胞水平和血管内皮生长因子、诱导型一氧化氮合酶及CD34水平，并进行比较。结果暴露后即刻组和暴露后1 d组、暴露后7 d组的外周血内皮祖细胞水平均显著高于空白对照组，差异有统计学意义(P<0.05)；但暴露后14 d组与空白对照组比较，差异无统计学意义(P>0.05)。暴露后1 d组、暴露后7 d组的血管内皮生长因子表达水平均显著高于空白对照组，差异有统计学意义(P<0.05)；但暴露后即刻组、暴露后14 d组与空白对照组比较，差异无统计学意义(P>0.05)。免疫组化结果显示：实验组噪声暴露后即刻、暴露后l d的CD34均呈微弱表达，噪声暴露后7 d呈强表达；噪声暴露后14 d呈阳性表达；噪声暴露后即刻的诱导型一氧化氮合酶呈微弱表达，暴露后1 d呈强表达，暴露后7、14 d组呈阳性表达。经扫描电镜结果显示：空白对照组动物的内耳内外毛细胞均呈现出整齐排列的情况，暴露后即刻组、暴露后1d组可见内耳毛细胞呈现出紊乱状态，暴露后7d组、暴露后14 d组，经观察动物耳蜗第二圈可见外毛细胞呈现出紊乱、缺失等现象。结论噪声性耳聋会导致耳蜗损伤的出现，内皮祖细胞数量会随着时间的变化发生改变，并在耳蜗损伤修复中发挥重要作用。
목적：탐토내피조세포재조성성이롱이와손상중적변화。방법50지SD대서분위량조，기중공백대조조10지，실험조40지。공백대조조불주처리，실험조진행조성폭로구건조성성이롱동물모형，병안조폭로시간분위4조，분별위폭로후즉각조화폭로후1 d조、폭로후7 d조、폭로후14 d조，매조각10지。분별검측각조동물적외주혈내피조세포수평화혈관내피생장인자、유도형일양화담합매급CD34수평，병진행비교。결과폭로후즉각조화폭로후1 d조、폭로후7 d조적외주혈내피조세포수평균현저고우공백대조조，차이유통계학의의(P<0.05)；단폭로후14 d조여공백대조조비교，차이무통계학의의(P>0.05)。폭로후1 d조、폭로후7 d조적혈관내피생장인자표체수평균현저고우공백대조조，차이유통계학의의(P<0.05)；단폭로후즉각조、폭로후14 d조여공백대조조비교，차이무통계학의의(P>0.05)。면역조화결과현시：실험조조성폭로후즉각、폭로후l d적CD34균정미약표체，조성폭로후7 d정강표체；조성폭로후14 d정양성표체；조성폭로후즉각적유도형일양화담합매정미약표체，폭로후1 d정강표체，폭로후7、14 d조정양성표체。경소묘전경결과현시：공백대조조동물적내이내외모세포균정현출정제배렬적정황，폭로후즉각조、폭로후1d조가견내이모세포정현출문란상태，폭로후7d조、폭로후14 d조，경관찰동물이와제이권가견외모세포정현출문란、결실등현상。결론조성성이롱회도치이와손상적출현，내피조세포수량회수착시간적변화발생개변，병재이와손상수복중발휘중요작용。
Objective To explore the changes of endothelial progenitor cells in noise deafness cochlea injure. Methods 50 SD rats were divided into two groups, the blank control group with 10 rats, and the experiment group with 40 rats. Blank control group received no intervention, experiment group exposed to noise and constructed noise deafness animal model, accoding to the exposed time, the rats were divided into four groups, after exposuring immediately group, after exposuring 1 d group, after exposuring 7 d group, after exposuring 14 d group, with 10 rats in each group. The periph-eral blood endothelial progenitor cells, blood vascular endothelial growth factor, inducible nitric oxide synthase and CD34 of animals were detected and compared. Results The peripheral blood endothelial progenitor cells in after expo-suring immediately group, after exposuring 1 d group and after exposuring 7 d group were higher than that in blank control group, the differences were statistically significant (P< 0.05); But there was no significant difference between after exposuring 14 d group and blank control group (P>0.05). The vascular endothelial growth factor expression levels in after exposuring 1 d group, after exposuring 7 d group were higher than that in blank control group, the differences were statistically significant (P<0.05);But those in after exposuring immediate group and after exposuring 11 d group were compared with those in blank control group, the differences were not statistically significant (P>0.05). The result of immunohistochemistry showed: in experiment group, after exposuring immediately and 1 d, expression of CD34 was weak, after exposuring 7 d was strong, after exposuring 14 d was positive; Expression of inducible nitric oxide synthaseafter exposuring immediately was weak, after exposuring 1 d was strong, after exposuring 7, 14 d was positive. The re-sults of scanning electron microscopy showed: the inner ear and inner hair cells of animal in the blank control group showed a neatly arranged, inner ear hair cells in after exposuring immediately group and after exposuring 1 d group showed a state of disorder, cochlea second ring visible outer hair cells in after exposuring 7 d group, after exposuring 14 d group showed lack of disorder phenomenon. Conclusion Noise deafness will lead cochlear injury, the number of endothelial progenitor cells will change with the change of time, and it plays an important role in cochlear injury repair.