CAJ | 학술논문

目的：探讨枸杞多糖（LBP）通过诱导巨噬细胞极化成一型巨噬细胞（M1）抗小鼠胰腺癌细胞LTPA的功效。方法构建小鼠CB-17SCID皮下LTPA成瘤模型，随机分为荷瘤模型组（10只）及LBP治疗组（10只），LBP治疗组每日10 mg/kg LBP灌胃，荷瘤模型组每日同等剂量的生理盐水灌胃。将含有相同数量的小鼠巨噬细胞Raw264.7随机分为不同LBP浓度的实验组和对照组，MTT法检测各实验组与对照组中Raw264.7的光密度（OD）值；ELISA法检测LBP质量浓度为100 mg/L的实验组与对照组中Raw264.7分泌白细胞介素（IL）-12及IL-10的水平；流式细胞仪检测LBP质量浓度为100 mg/L的实验组与对照组中Raw264.7表面蛋白CD16/32及CD206的水平。小鼠荷瘤3周后剖瘤称质量并计算瘤体体积，检测LBP对皮下肿瘤生长的影响，HE染色和KI-67免疫组化法检测LBP对瘤组织镜下的改变及对LTPA增殖的影响。结果100 mg/L LBP对Raw264.7的生长有明显促进作用（P<0.01），并使Raw264.7高表达CD16/32，低表达CD206；高分泌IL-12、低分泌IL-10。LBP治疗组瘤块质量、体积及KI-67的表达量显著低于荷瘤模型组（P<0.01），镜下肿瘤坏死区范围明显大于荷瘤模型组。结论 LBP能够通过诱导巨噬细胞极化成M1状态达到抗LTPA的作用。
목적：탐토구기다당（LBP）통과유도거서세포겁화성일형거서세포（M1）항소서이선암세포LTPA적공효。방법구건소서CB-17SCID피하LTPA성류모형，수궤분위하류모형조（10지）급LBP치료조（10지），LBP치료조매일10 mg/kg LBP관위，하류모형조매일동등제량적생리염수관위。장함유상동수량적소서거서세포Raw264.7수궤분위불동LBP농도적실험조화대조조，MTT법검측각실험조여대조조중Raw264.7적광밀도（OD）치；ELISA법검측LBP질량농도위100 mg/L적실험조여대조조중Raw264.7분비백세포개소（IL）-12급IL-10적수평；류식세포의검측LBP질량농도위100 mg/L적실험조여대조조중Raw264.7표면단백CD16/32급CD206적수평。소서하류3주후부류칭질량병계산류체체적，검측LBP대피하종류생장적영향，HE염색화KI-67면역조화법검측LBP대류조직경하적개변급대LTPA증식적영향。결과100 mg/L LBP대Raw264.7적생장유명현촉진작용（P<0.01），병사Raw264.7고표체CD16/32，저표체CD206；고분비IL-12、저분비IL-10。LBP치료조류괴질량、체적급KI-67적표체량현저저우하류모형조（P<0.01），경하종류배사구범위명현대우하류모형조。결론 LBP능구통과유도거서세포겁화성M1상태체도항LTPA적작용。
Objective To explore the effects of lycium barbarum polysaccharide (LBP) on restraining the mouse pancre?atic cancer cells LTPA by the polarization of macrophages to type 1 macrophages (M1). Methods LTPA tumor model of the subcutaneous CB-17SCID mice was constructed. Model mice were randomly divided into tumor-bearing model group (n=10) and LBP treatment group (n=10). The LBP treatment group was fed 10mg/kg LBP every day, and the tumor-bearing model group was fed the same dose of normal saline. The same amount of macrophages Raw264.7 was randomly divided into the control group and experimental groups (different concentrations of LBP). MTT assay was used to detect the optical density (OD) of Raw264.7 in experimental groups and control group. ELISA was used to detect the levels of the interleukin (IL)-12 and IL-10 in experimental group (LBP was 100 mg/L) and the control group. Flow cytometry was used to test the levels of the membrane protein CD16/32 and CD206 in experimental group (LBP was 100 mg/L) and the control group. The tumor mass was weighted and the volume was calculated after three weeks. The effects of LBP on the growth of subcutaneous tumor were detected. HE staining and KI-67 staining were used to detect the microscopic changes of tumor and the proliferation of the LTPA. Results The dose of 100 mg/L LBP can promote the growth of the macrophages Raw264.7 (P<0.01), and induced the high expression of CD16/32 and low expression of CD206, high secretion of IL-12 and low secretion of IL-10. The weight, volume of the tumor and the expression of KI-67 were significantly lower in experimental group than those in the con?trol group (P<0.01). The microscopic necrosis area range of tumor was larger than that of control group. Conclusion The LBP has the effect of restraining LTPA by the polarization of macrophages to M1.