CAJ | 학술논문

目的：研究裙带菜内生菌Bac illus sp. QD-4发酵液的化学成分。方法采用硅胶柱层析、羟丙基葡聚糖凝胶柱层析、薄层层析、反相高效液相色谱等方法对QD-4发酵液乙酸乙酯提取物进行分离纯化，并采用质谱、核磁共振谱等技术及文献比对的方法对化合物进行结构鉴定；采用改良Ellman法测定化合物对乙酰胆碱酯酶(AChE)的抑制活性。结果从QD-4发酵液乙酸乙酯提取物中分离得到5种化合物：环-(L-亮氨酸-L-4-羟脯氨酸)(1)、环-(L-脯氨酸-L-酪氨酸)(2)、环-(甘氨酸-L-脯氨酸)(3)、环-(L-脯氨酸-D-酪氨酸)(4)和尿嘧啶(5)。在400μg/mL浓度下，化合物2和4对AChE的抑制率分别为(11.62±2.29)%和(5.62±1.63)%。结论化合物2和4对AchE具有一定的抑制活性，是Bacillus sp. QD-4发酵液抑制AChE活性的物质基础。
목적：연구군대채내생균Bac illus sp. QD-4발효액적화학성분。방법채용규효주층석、간병기포취당응효주층석、박층층석、반상고효액상색보등방법대QD-4발효액을산을지제취물진행분리순화，병채용질보、핵자공진보등기술급문헌비대적방법대화합물진행결구감정；채용개량Ellman법측정화합물대을선담감지매(AChE)적억제활성。결과종QD-4발효액을산을지제취물중분리득도5충화합물：배-(L-량안산-L-4-간포안산)(1)、배-(L-포안산-L-락안산)(2)、배-(감안산-L-포안산)(3)、배-(L-포안산-D-락안산)(4)화뇨밀정(5)。재400μg/mL농도하，화합물2화4대AChE적억제솔분별위(11.62±2.29)%화(5.62±1.63)%。결론화합물2화4대AchE구유일정적억제활성，시Bacillus sp. QD-4발효액억제AChE활성적물질기출。
Objective To study chemical constituents of liquid culture of endophyte Bac illus sp. QD-4 from Undaria pinnatifida. Methods Isolation and purification of EtOAc extract of liquid culture of QD-4 was performed through silica gel chromatograph, gel filtration over Sephadex LH-20, TLC and RP-HPLC and structures of obtained compounds were elucidated by spectral techniques such as mass spectrum, NMR spectra and comparison with literatures. Acetylc-holesterase (AChE) inhibitory activity of compounds was evaluated by modified Ellman method. Results Five com-pounds were isolated from the EtOAc extract of liquid culture of strain QD-4 and identified as cyclo-(L-Leu-L-4-hy-droxy-Pro) (1), cyclo-(L-Pro-L-Tyr) (2), cyclo-(Gly-L-Pro) (3), cyclo-(L-Pro-D-Tyr) (4) and uracil (5) respectively. At concentration of 400 μg/mL, compounds 2 and 4 were found to inhibit acetylcholesterase with inhibition rate of (11.62±2.29)% and (5.62±1.63)%respectively. Conclusion Compounds 2 and 4 can inhibit acetylcholesterase to some extent, which is material basis of liquid culture of Bacillus sp. QD-4 for AChE inhibitory activity.