CAJ | 학술논문

目的：研究杨梅素(Myr)体内外对人肝癌Bel-7402细胞增殖、凋亡及机制的影响。方法体外培养人肝癌Bel-7402细胞，MTT和SRB法检测细胞增殖抑制率；采用25只SPF级BALB/C-nu裸鼠建立Bel-7402细胞裸鼠异种移植瘤模型，分为5组，对照组，5-氟尿嘧啶(5-Fu，25 mg/kg)阳性对照组以及Myr(20、40、80 mg/kg)三个剂量组。腹腔注射给药3周后，处死裸鼠，剥瘤称重并计算抑瘤率。TUNEL法检测肿瘤组织细胞凋亡；FACS检测肿瘤组织细胞周期；免疫组织化学法检测Bax和Bcl-2含量。结果 MTT和 SRB法检测显示，Myr可以抑制Bel-7402细胞体外增殖并呈剂量依赖。腹腔注射给药3周后，Myr各剂量组瘤质量与模型组比较，差异有高度统计学意义(P<0.01)；Myr体各剂量组Bel-7402细胞凋亡率与对照组比较，差异有高度统计学意义(P<0.01)，Myr将细胞周期阻滞在G2~M期。 Myr可以促进Bax蛋白表达(P<0.05或P<0.01)，抑制Bcl-2蛋白表达(P<0.05或P<0.01)。结论杨梅素可抑制Bel-7402细胞的增殖，诱导其凋亡，其凋亡机制涉及Bcl-2家族。
목적：연구양매소(Myr)체내외대인간암Bel-7402세포증식、조망급궤제적영향。방법체외배양인간암Bel-7402세포，MTT화SRB법검측세포증식억제솔；채용25지SPF급BALB/C-nu라서건립Bel-7402세포라서이충이식류모형，분위5조，대조조，5-불뇨밀정(5-Fu，25 mg/kg)양성대조조이급Myr(20、40、80 mg/kg)삼개제량조。복강주사급약3주후，처사라서，박류칭중병계산억류솔。TUNEL법검측종류조직세포조망；FACS검측종류조직세포주기；면역조직화학법검측Bax화Bcl-2함량。결과 MTT화 SRB법검측현시，Myr가이억제Bel-7402세포체외증식병정제량의뢰。복강주사급약3주후，Myr각제량조류질량여모형조비교，차이유고도통계학의의(P<0.01)；Myr체각제량조Bel-7402세포조망솔여대조조비교，차이유고도통계학의의(P<0.01)，Myr장세포주기조체재G2~M기。 Myr가이촉진Bax단백표체(P<0.05혹P<0.01)，억제Bcl-2단백표체(P<0.05혹P<0.01)。결론양매소가억제Bel-7402세포적증식，유도기조망，기조망궤제섭급Bcl-2가족。
Objective To study the influence of Myricetin (Myr) on human hepatoma Bel-7402 cell multiplication, apoptosis and its mechanism in vivo and in vitro. Methods Bel-7402 cells were cultured in vitro, MTT and SRB were performed to observe the proliferation. The 25 BALB/C-nu nude mice of SPF lever were made Bel-7402 cell xenografts model, and they were divided into five groups, control group, 5-Fu (25 mg/kg) positive control group, Myr (20, 40, 80 mg/kg) three doses groups. After 3 weeks drug abdominal injection, killed mice, the tumor was weighed and tumor-inhibition rate was counted. TUNEL was used for measuring the apoptosis, FACS was used for measuring the cell cycle;Immuno-histochemistry was used for detecting Bax and Bcl-2 content. Results By MTT and SRB detection, Myr could inhibit Bel-7402 cell proliferation in v itro, and it showed dosage dependent. After 3 weeks drug abdominal injection, tumor mass of Myr (20, 40, 80 mg/kg) three doses groups were compared with control group, the differences were statistically significant (P<0.01);apoptosis rate of Bel-7402 cell of Myr (20, 40, 80 mg/kg) three doses groups were compared with control group, the differences were statistically significant (P<0.01), Myr arrested cell cycle in G2-M phase. Myr pro-moted Bax expression (P< 0.05 or P< 0.01), and inhibited Bcl-2 expression (P< 0.05 or P< 0.01). Conclusion Myr can inhibit the proliferation of Bel-7402 cells and induce apoptosis, the mechanism involves Bcl-2 family.