河北渔业
河北漁業
하북어업
Hebei Fisheries
2015年
11期
4-7,41
,共5页
宋淑梅%马睿霄%金枫清%佟长青%翟兴月%李伟
宋淑梅%馬睿霄%金楓清%佟長青%翟興月%李偉
송숙매%마예소%금풍청%동장청%적흥월%리위
双齿围沙蚕%化学成分%分离纯化%结构鉴定%抑制肿瘤细胞活性
雙齒圍沙蠶%化學成分%分離純化%結構鑒定%抑製腫瘤細胞活性
쌍치위사잠%화학성분%분리순화%결구감정%억제종류세포활성
Perinereis aibuhitensis%constituent%structural identification%tumour cell
采用硅胶柱色谱及薄层制备色谱等手段从双齿围沙蚕(Perinereis aibuhitensis)中分离纯化出2种化合物。对所获得的化合物通过理化常数测定、波谱数据分析同文献对照的方法进行了结构鉴定。同时,采用CCK -8方法对双齿围沙蚕浸膏及乙酸乙酯萃取部分分别进行抑制肿瘤细胞活性测试。结果表明,从双齿围沙蚕中分离得到2种化合物分别为:胆甾-4烯3α,6β醇(1)和(E)-17-(4,7-dimethyloct-5-en-2-yl)-10,13-dimethyl-2,3,4,7,8,9,10,11,12,13,14,15,16,17-tetradecahydro -1H -cyclopenta[a]phenan‐thren-3-ol(2),且化合物1是首次从沙蚕中分离得到。抑制肿瘤细胞活性测试表明,70%乙醇浸提获得的双齿围沙蚕浸膏及乙酸乙酯萃取部分对人胃癌细胞AGS、人肝癌细胞 HepG2以及人卵巢癌细胞SKOV3均具有抑制细胞增殖作用,且具有剂量和时间依赖性,可作为潜在的抗肿瘤药物进行进一步研究。
採用硅膠柱色譜及薄層製備色譜等手段從雙齒圍沙蠶(Perinereis aibuhitensis)中分離純化齣2種化閤物。對所穫得的化閤物通過理化常數測定、波譜數據分析同文獻對照的方法進行瞭結構鑒定。同時,採用CCK -8方法對雙齒圍沙蠶浸膏及乙痠乙酯萃取部分分彆進行抑製腫瘤細胞活性測試。結果錶明,從雙齒圍沙蠶中分離得到2種化閤物分彆為:膽甾-4烯3α,6β醇(1)和(E)-17-(4,7-dimethyloct-5-en-2-yl)-10,13-dimethyl-2,3,4,7,8,9,10,11,12,13,14,15,16,17-tetradecahydro -1H -cyclopenta[a]phenan‐thren-3-ol(2),且化閤物1是首次從沙蠶中分離得到。抑製腫瘤細胞活性測試錶明,70%乙醇浸提穫得的雙齒圍沙蠶浸膏及乙痠乙酯萃取部分對人胃癌細胞AGS、人肝癌細胞 HepG2以及人卵巢癌細胞SKOV3均具有抑製細胞增殖作用,且具有劑量和時間依賴性,可作為潛在的抗腫瘤藥物進行進一步研究。
채용규효주색보급박층제비색보등수단종쌍치위사잠(Perinereis aibuhitensis)중분리순화출2충화합물。대소획득적화합물통과이화상수측정、파보수거분석동문헌대조적방법진행료결구감정。동시,채용CCK -8방법대쌍치위사잠침고급을산을지췌취부분분별진행억제종류세포활성측시。결과표명,종쌍치위사잠중분리득도2충화합물분별위:담치-4희3α,6β순(1)화(E)-17-(4,7-dimethyloct-5-en-2-yl)-10,13-dimethyl-2,3,4,7,8,9,10,11,12,13,14,15,16,17-tetradecahydro -1H -cyclopenta[a]phenan‐thren-3-ol(2),차화합물1시수차종사잠중분리득도。억제종류세포활성측시표명,70%을순침제획득적쌍치위사잠침고급을산을지췌취부분대인위암세포AGS、인간암세포 HepG2이급인란소암세포SKOV3균구유억제세포증식작용,차구유제량화시간의뢰성,가작위잠재적항종류약물진행진일보연구。
Two compounds were isolated from Perinereis aibuhitensis by column chromatography on silica gel and thin layer chromatography .Their structures were identified by spectroscopic analysis and physical-chemistry properties .The inhibition on tumour cells of all extracts from P .aibuhitensis were evaluated by CCK -8 assay .The results showed that two compounds were obtained and identified as cholest -4-ene-3α,6β-diol and (E)-17 -(4 ,7 -dimethyloct -5 -en-2yl)-10 ,13 -dimethyl -2 ,3 ,4 ,7 ,8 ,9 ,10 ,11 ,12 ,13 ,14 ,15 ,16 ,17-tetradecahydro -1H -cyclopenta [a] phenanthren-3-ol . All extracts from P .aibuhitensis using 75% EtOH and ethyl acetate layer exhibited the potent inhibi‐tory activities against HepG2 ,AGS and SKOV3 cells .The extracts could be considered as potential an‐ticancer candidate for further study .