CAJ | 학술논문

目的：比较两种不同方法提取冻存全血中基因组DNA的效果和特点。方法分别用改良碘化钾法和Promega全血基因组提取试剂盒提取全血基因组DNA，通过紫外分光光度仪、凝胶电泳、PCR进行检测。结果改良碘化钾法和试剂盒法提取的基因组DNA浓度分别为（330.9±0.94）ng/μL和（490.3±3.16）ng/μL；纯度为（1.87±0.03）和（1.85±0.06）。试剂盒法提取的基因组DNA含量稍高于改良碘化钾法，质量和纯度无显著差异。结论试剂盒法更简便、快速、无毒地进行提取DNA，但价格较贵；改良碘化钾法价格低廉，适合大量临床血液标本的提取，能够满足分子生物学实验的需要。
목적：비교량충불동방법제취동존전혈중기인조DNA적효과화특점。방법분별용개량전화갑법화Promega전혈기인조제취시제합제취전혈기인조DNA，통과자외분광광도의、응효전영、PCR진행검측。결과개량전화갑법화시제합법제취적기인조DNA농도분별위（330.9±0.94）ng/μL화（490.3±3.16）ng/μL；순도위（1.87±0.03）화（1.85±0.06）。시제합법제취적기인조DNA함량초고우개량전화갑법，질량화순도무현저차이。결론시제합법경간편、쾌속、무독지진행제취DNA，단개격교귀；개량전화갑법개격저렴，괄합대량림상혈액표본적제취，능구만족분자생물학실험적수요。
ObjectiveTo compare the effect and characteristics of genomic DNA extraction from frozen whole blood using two different methods. MethodsGenomic DNA were extracted with potassium iodide method and Promega Genomic DNA Puriifcation Kit.The detection was performed by UV spectrophotometry, gel electrophoresis and PCR.ResultsThe concentration of genomic DNA extracted by potassium iodide method and Promega Genomic DNA Puriifcation Kit method was: (330.9±0.94)ng/μL and (490.3±3.16)ng/μL; and the ratio of A260/A280 was: (1.87±0.03) and (1.85±0.06).The quantity of genomic DNA extracted by kit is slightly higher than that by potassium iodide method. There is no signiifcant difference in DNA quality and purity between two methods.ConclusionKit is the more simple, faster, non-toxic method compared with the potassium iodide method, but the cost is expensive. Potassium iodide method is helpful to extract genomic DNA from a large number of clinical blood samples due to the low cost. Two methods are able to meet the needs of molecular biology experiments.