CAJ | 학술논문

目的：研究依折麦布和辛伐他汀单独及联合使用对模拟缺血再灌注大鼠心肌细胞瞬时外向钾电流（Ito）的影响。方法将75只雄性SD大鼠按随机数字表法分为正常对照（CON）组、模拟缺血再灌注（CIR）组、依折麦布（EIR）组、辛伐他汀（SIR）组和依折麦布+辛伐他汀（ESIR）组，分别予生理盐水或其溶解的药物灌胃2周后分离右室心肌细胞，采用全细胞膜片钳技术记录Ito。结果（1）Ito电流密度（+60 mV）：CIR组较CON组下降，EIR组与CIR组比较差异无统计学意义（P＞0.05），SIR组、ESIR组与CIR组比较均升高，SIR组与ESIR组比较差异无统计学意义（P＞0.05）。（2）Ito稳态失活曲线最大半数失活电压（V1/2）：CIR组较CON组显著增大，EIR组与CIR组比较差异无统计学意义（P＞0.05），SIR组和ESIR组较CIR组均显著减小（P＜0.05），SIR组和ESIR组比较差异无统计学意义（P＞0.05）。斜率因子（K）各组间差异无统计学意义（P＞0.05）。（3）失活后恢复曲线失活时间常数（τ）值：CIR组较CON组显著增加，EIR组与CIR组间差异无统计学意义（P＞0.05），SIR组、ESIR组较CIR组明显减低（P＜0.05），而SIR组与ESIR组比较差异无统计学意义（P＞0.05）。结论辛伐他汀、依折麦布+辛伐他汀预处理可以逆转模拟缺血再灌注对心肌细胞Ito的影响，且效果相似，而依折麦布未表现出这种效果。
목적：연구의절맥포화신벌타정단독급연합사용대모의결혈재관주대서심기세포순시외향갑전류（Ito）적영향。방법장75지웅성SD대서안수궤수자표법분위정상대조（CON）조、모의결혈재관주（CIR）조、의절맥포（EIR）조、신벌타정（SIR）조화의절맥포+신벌타정（ESIR）조，분별여생리염수혹기용해적약물관위2주후분리우실심기세포，채용전세포막편겸기술기록Ito。결과（1）Ito전류밀도（+60 mV）：CIR조교CON조하강，EIR조여CIR조비교차이무통계학의의（P＞0.05），SIR조、ESIR조여CIR조비교균승고，SIR조여ESIR조비교차이무통계학의의（P＞0.05）。（2）Ito은태실활곡선최대반수실활전압（V1/2）：CIR조교CON조현저증대，EIR조여CIR조비교차이무통계학의의（P＞0.05），SIR조화ESIR조교CIR조균현저감소（P＜0.05），SIR조화ESIR조비교차이무통계학의의（P＞0.05）。사솔인자（K）각조간차이무통계학의의（P＞0.05）。（3）실활후회복곡선실활시간상수（τ）치：CIR조교CON조현저증가，EIR조여CIR조간차이무통계학의의（P＞0.05），SIR조、ESIR조교CIR조명현감저（P＜0.05），이SIR조여ESIR조비교차이무통계학의의（P＞0.05）。결론신벌타정、의절맥포+신벌타정예처리가이역전모의결혈재관주대심기세포Ito적영향，차효과상사，이의절맥포미표현출저충효과。
Objective To observe the impact of alone or combined use of ezetimibe and simvastatin on transient outward potassium current (Ito) in ventricular myocytes of rat model of ischemia and reperfusion (IR). Methods Seventy-five male SD rats were randomly divided into five groups, control group (CON), control-IR group (CIR), ezetimibe treatment group (EIR), simvastatin treatment group (SIR) and combined ezetimibe and simvastatin treatment group (ESIR). After two weeks of treat?ment with intragastic normal saline or drugs (ezetimibe or simvastatin), myocytes were isolated from right ventricular with colla?genaseⅡ, and Ito was recorded by whole-cell patch clamp technique. Results (1) The Ito current density at+60 mV was sig?nificantly decreased in CIR group than that of CON group (P<0.05). There was no significant difference in Ito current density between SIR group and ESIR group (P>0.05). The Ito current densities were higher in SIR group and ESIR group compared to those of CIR group. There was no significant difference in Ito current density between SIR group and ESIR group (P>0.05). (2) There was a significant increase in the half-inactivation (V1/2) in CIR group than that of CON group, but no significant differ?ence between EIR group and CIR group (P >0.05). There was a significant difference in the half-inactivation (V1/2) in SIR group and ESIR group compared to that of CIR group (P<0.05), but no significant difference between SIR group and ESIR group (P>0.05). There was no significant difference in the slope factor (K) between five groups (P>0.05). (3) The time-con?stant (τ) of Ito recovery curves from inactivation was significantly higher in CIR group than that of CON group (P<0.05), which was no significant difference between EIR group and CIR group (P>0.05). There was a significant difference in the time-con?stant (τ) of Ito recovery curves from inactivation in SIR group and ESIR group compared to that of CIR group (P<0.05), but no significant difference between SIR group and ESIR group (P>0.05). Conclusion Simvastatin pre-treatment or ezetimibe+simvastatin pre-treatment can reverse the effect of IR on Ito of ventricular myocytes, but ezetimibe shows no such effects.