CAJ | 학술논문

目的：探讨免疫耐受疫苗技术是否可以通过调节T细胞抑制炎性T细胞引起的过敏性哮喘的发生。方法 BALB／c小鼠随机分成5组，每组10只。各组用不同的策略免疫小鼠，具体为：第1组小鼠注射100μl磷酸盐缓冲液（ PBS）为阴性对照组；第2组小鼠注射10μg卵白蛋白；第3组小鼠注射10μg地塞米松；第4组小鼠将10μg卵白蛋白与10μg地塞米松共同注射；第5组小鼠将100μg卵白蛋白与100μg地塞米松共同注射。各组均于第1天、4天、7天和14天通过背部皮下注射的方式进行免疫小鼠。在最后一次免疫后7 d，对各组小鼠同时诱导过敏性哮喘，24 h后，评价各组小鼠的哮喘发病情况，评价指标包括：肺部病理变化、支气管肺泡灌洗液中炎性细胞的浸润、血清抗体的产生、过敏反应试验等。以此评价免疫耐受疫苗对小鼠哮喘发病的影响。结果免疫耐受性疫苗免疫的小鼠肺组织炎症细胞浸润、血清中IgE和IgG1抗体水平、肺组织CD4＋T细胞的浸润程度等都有了显著降低，而CD4＋CD25＋Foxp3＋的调节性T 细胞所占CD4＋T细胞中的比例明显增高。同时，耐受性疫苗能够抑制Th2型细胞因子的表达。结论耐受性疫苗免疫能够诱导调节性T细胞产生并影响炎性T细胞及其淋巴细胞因子的表达，从而抑制小鼠哮喘的发生。
목적：탐토면역내수역묘기술시부가이통과조절T세포억제염성T세포인기적과민성효천적발생。방법 BALB／c소서수궤분성5조，매조10지。각조용불동적책략면역소서，구체위：제1조소서주사100μl린산염완충액（ PBS）위음성대조조；제2조소서주사10μg란백단백；제3조소서주사10μg지새미송；제4조소서장10μg란백단백여10μg지새미송공동주사；제5조소서장100μg란백단백여100μg지새미송공동주사。각조균우제1천、4천、7천화14천통과배부피하주사적방식진행면역소서。재최후일차면역후7 d，대각조소서동시유도과민성효천，24 h후，평개각조소서적효천발병정황，평개지표포괄：폐부병리변화、지기관폐포관세액중염성세포적침윤、혈청항체적산생、과민반응시험등。이차평개면역내수역묘대소서효천발병적영향。결과면역내수성역묘면역적소서폐조직염증세포침윤、혈청중IgE화IgG1항체수평、폐조직CD4＋T세포적침윤정도등도유료현저강저，이CD4＋CD25＋Foxp3＋적조절성T 세포소점CD4＋T세포중적비례명현증고。동시，내수성역묘능구억제Th2형세포인자적표체。결론내수성역묘면역능구유도조절성T세포산생병영향염성T세포급기림파세포인자적표체，종이억제소서효천적발생。
Objective To investigate whether immunization mice with an immune tolerogenic vac-cine could inhibit the occurrence of allergic asthma through regulating the inflammatory T cells . Methods The BALB/c mice were randomly divided into 5 groups, with 10 mice per group.The mice in different groups were treated with different immunization strategies , which were 100 μl of phosphate buffer ( PBS) for the negative control , 10 μg of ovalbumin for the unrelated antigen control , 10 μg of dexametha-sone for group 3, 10μg of ovalbumin protein and 10μg dexamethasone for group 4 and 100 μg of ovalbumin protein and 100 μg of dexamethasone for group 5.The mice were immunized subcutaneously on days 1, 4, 7, and 14.Seven days after the last immunization , all mice were used for the induction of allergic asthma . The incidences of asthma in mice from different groups were evaluated 24 hours after the induction .The eval-uation indicators included pathological changes in lung tissues , infiltration of inflammatory cells in bronchial alveolar lavage fluid , antibody in serum samples and allergic responses .Results Immunization mice with the immune tolerogenic vaccine significantly reduced the infiltration of inflammatory cells in lung tissues , de-creased the levels of IgE and IgG 1 antibodies in serum samples and alleviated the injuries and pathological changes in lung tissues.However, the percentages of CD4+CD25+Foxp3+regulatory T cells to CD4+T popu-lations were significantly increased .Moreover, immunization mice with the tolerogenic vaccine could inhibit the expression of Th2 type cytokines .Conclusion Immunization mice with the tolerogenic vaccine could in-duce high levels of regulatory T cells , reduce the infiltration of inflammatory T cells in lung tissues and in-hibit the expression of Th2 cytokines, resulting in the inhibited occurrence of asthma in the murine model .