CAJ | 학술논문

[目的]建立冬虫夏草HPLC指纹图谱，为其真伪鉴别提供有效方法，并定量测定其6种共有核苷类成分（尿嘧啶、尿苷、胸腺嘧啶、腺嘌呤、腺苷、虫草素）的质量。[方法]采用HPLC，色谱柱Waters Symmetry RP-C18（250×4.6mm，5μm），以乙酸缓冲盐（pH 6.5，A）-甲醇（B）为流动相，梯度洗脱，检测波长260 nm，柱温25℃，流速0.8 mL/min。应用中药色谱指纹图谱相似度计算软件对指纹图谱进行相似度评价、聚类分析。[结果]所建立的方法重复性和精密度良好，建立的冬虫夏草指纹图谱包含24个共有峰，各峰分离良好，确认出尿嘧啶、尿苷、胸腺嘧啶、腺嘌呤、腺苷、虫草素6个共有成分。考察了29批西藏冬虫夏草，3批西藏以外地区冬虫夏草，2批蛹虫草，1批假虫草的指纹图谱与冬虫夏草指纹对照图谱的相似度。通过相似度分析，能很好的区分冬虫夏草、蛹虫草和假虫草。[结论]所建立的方法简单可靠，可做为冬虫夏草真伪鉴定的有效实验方法和依据。
[목적]건입동충하초HPLC지문도보，위기진위감별제공유효방법，병정량측정기6충공유핵감류성분（뇨밀정、뇨감、흉선밀정、선표령、선감、충초소）적질량。[방법]채용HPLC，색보주Waters Symmetry RP-C18（250×4.6mm，5μm），이을산완충염（pH 6.5，A）-갑순（B）위류동상，제도세탈，검측파장260 nm，주온25℃，류속0.8 mL/min。응용중약색보지문도보상사도계산연건대지문도보진행상사도평개、취류분석。[결과]소건립적방법중복성화정밀도량호，건립적동충하초지문도보포함24개공유봉，각봉분리량호，학인출뇨밀정、뇨감、흉선밀정、선표령、선감、충초소6개공유성분。고찰료29비서장동충하초，3비서장이외지구동충하초，2비용충초，1비가충초적지문도보여동충하초지문대조도보적상사도。통과상사도분석，능흔호적구분동충하초、용충초화가충초。[결론]소건립적방법간단가고，가주위동충하초진위감정적유효실험방법화의거。
Objective] To establish HPLC fingerprints for indentification ofCordycepssinensisin Tibet. [Methods] The HPLC analysis was performed on Waters Symmetry RP-C18 column (250×4.6mm, 5μm) and detected at 260nm. The mobile phase was consisted of methnol and acetate buffer (pH 6.5) using a gradient elution. The column temperature was 25℃, and the flow rate was 0.8mL/min. The similarity of fingerprints was evaluated by the fingerprint similarity calculation software, then, duster analysis was studied. [Results] The precision and repeatability of the method were good as the relative standard deviation (RSD) of intrabatch was less than 5%. The fingerprints of 29 batches ofCordycepssinensisin Tibet, 3Cordycepssinensisfrom Sichuan and Qinghai, 2Cordycepsmilitaris, and 1 fakeCordyceps sinensiswere compared in characteristic components and similarity. The results demonstrated that Cordycepssinensiswas differentiated from theCordycepsmilitarisand fakeCordycepssinensis. [Conclusion] The method was simple, feasible and reproducible, and can be offered as technical basis for the identification ofCordycepssinensis.