中华微生物学和免疫学杂志
中華微生物學和免疫學雜誌
중화미생물학화면역학잡지
Chinese Journal of Microbiology and Immunology
2015年
9期
698-702
,共5页
长链非编码RNA%NEAT1%MALAT1%人类免疫缺陷病毒-1%高效抗逆转录病毒治疗
長鏈非編碼RNA%NEAT1%MALAT1%人類免疫缺陷病毒-1%高效抗逆轉錄病毒治療
장련비편마RNA%NEAT1%MALAT1%인류면역결함병독-1%고효항역전록병독치료
Long non-coding RNA%Nuclear-enriched autosomal transcript 1 ( NEAT1)%Metasta-sis associated lung adenocarcinoma transcript 1 ( MALAT1 )%HIV-1%Highly active antiretroviral therapy
目的：探讨HIV-1感染者长链非编码RNA（lncRNA） NEAT1和MALAT1的水平与HIV-1感染患者疾病进展的关系。方法募集59名HIV-1感染者与21名HIV-1阴性健康对照者，其中HIV-1感染者中31人未接受高效抗逆转录病毒治疗（HAART），28人接受HAART治疗1年以上并且病毒载量低于检测限。检测CD4＋T细胞数；分离外周血单个核细胞（ PBMC）和血浆，分别提取总RNA后，用实时荧光定量PCR法检测NEAT1和MALAT1的水平，分析其对疾病进展的影响。结果在未接受HAART的HIV-1感染者的PBMC中，NEAT1和MALAT1的水平显著高于正常水平，为正常水平的3～5倍（P＜0．01）；而病毒载量被HAART有效抑制的治疗者的PBMC中，NEAT1和MALAT1的水平显著低于未治疗组（P＜0．01），接近正常水平。两组HIV-1感染者血浆中NEAT1的水平显著低于对照组（P＜0．01），并且与CD4＋T细胞成正相关（P＜0．01），而MALAT1差异不明显（P＞0．05）。结论 NEAT1和MALAT1可能在人体HIV-1感染中对疾病的进展发挥一定的作用，并且血浆中的NEAT1水平可以作为一种潜在的HIV-1感染病程进展的生物标记物。
目的：探討HIV-1感染者長鏈非編碼RNA（lncRNA） NEAT1和MALAT1的水平與HIV-1感染患者疾病進展的關繫。方法募集59名HIV-1感染者與21名HIV-1陰性健康對照者，其中HIV-1感染者中31人未接受高效抗逆轉錄病毒治療（HAART），28人接受HAART治療1年以上併且病毒載量低于檢測限。檢測CD4＋T細胞數；分離外週血單箇覈細胞（ PBMC）和血漿，分彆提取總RNA後，用實時熒光定量PCR法檢測NEAT1和MALAT1的水平，分析其對疾病進展的影響。結果在未接受HAART的HIV-1感染者的PBMC中，NEAT1和MALAT1的水平顯著高于正常水平，為正常水平的3～5倍（P＜0．01）；而病毒載量被HAART有效抑製的治療者的PBMC中，NEAT1和MALAT1的水平顯著低于未治療組（P＜0．01），接近正常水平。兩組HIV-1感染者血漿中NEAT1的水平顯著低于對照組（P＜0．01），併且與CD4＋T細胞成正相關（P＜0．01），而MALAT1差異不明顯（P＞0．05）。結論 NEAT1和MALAT1可能在人體HIV-1感染中對疾病的進展髮揮一定的作用，併且血漿中的NEAT1水平可以作為一種潛在的HIV-1感染病程進展的生物標記物。
목적：탐토HIV-1감염자장련비편마RNA（lncRNA） NEAT1화MALAT1적수평여HIV-1감염환자질병진전적관계。방법모집59명HIV-1감염자여21명HIV-1음성건강대조자，기중HIV-1감염자중31인미접수고효항역전록병독치료（HAART），28인접수HAART치료1년이상병차병독재량저우검측한。검측CD4＋T세포수；분리외주혈단개핵세포（ PBMC）화혈장，분별제취총RNA후，용실시형광정량PCR법검측NEAT1화MALAT1적수평，분석기대질병진전적영향。결과재미접수HAART적HIV-1감염자적PBMC중，NEAT1화MALAT1적수평현저고우정상수평，위정상수평적3～5배（P＜0．01）；이병독재량피HAART유효억제적치료자적PBMC중，NEAT1화MALAT1적수평현저저우미치료조（P＜0．01），접근정상수평。량조HIV-1감염자혈장중NEAT1적수평현저저우대조조（P＜0．01），병차여CD4＋T세포성정상관（P＜0．01），이MALAT1차이불명현（P＞0．05）。결론 NEAT1화MALAT1가능재인체HIV-1감염중대질병적진전발휘일정적작용，병차혈장중적NEAT1수평가이작위일충잠재적HIV-1감염병정진전적생물표기물。
Objective To investigate the expression of two long non-coding RNAs ( lncRNAs ) during HIV-1 infection, which were nuclear-enriched autosomal transcript 1 (NEAT1) and metastasis asso-ciated lung adenocarcinoma transcript 1 ( MALAT1 ) , and their relationships with disease progression . Methods Fifty-nine patients with HIV-1 infection and 21 healthy subjects were recruited in this study , of which 31 patients were highly active antiretroviral therapy ( HAART)-na?ve and 28 patients received HAART for more than one year with undetectable viral loads .Total RNAs were extracted from PBMC and plasma samples, respectively.The levels of NEAT1 and MALAT1 were detected by quantitative real time polymer-ase chain reaction .Results The levels of NAET1 and MALAT1 in PBMC from HAART na?ve patients were 3 to 5 times higher than those in healthy subjects (P<0.01).The levels of NAET1 and MALAT1 in PBMC from HAART treated patients were similar to those in healthy subjects .The levels of NEAT1 in plasma sam-ples from patients with HIV-1 infection were lower than those in healthy subjects , and were positively corre-lated with CD4+T cell counts (P<0.01).No significance differences in the levels of MALAT1 in plasma samples were found between those with and without HIV-1 infection (P>0.05).Conclusion This study suggested that NEAT 1 and MALAT1 might be involved in the disease progression in patients with HIV-1 in-fection.The level of NEAT1 in plasma could be used as a potential biomarker of HIV-1 infection.
