临床神经病学杂志
臨床神經病學雜誌
림상신경병학잡지
Journal of Clinical Neurology
2015年
5期
371-374
,共4页
王倩倩%刘益民%钟平%张雷%王玉
王倩倩%劉益民%鐘平%張雷%王玉
왕천천%류익민%종평%장뢰%왕옥
癫痫%匹罗卡品%经皮三叉神经电刺激%Morris水迷宫试验
癲癇%匹囉卡品%經皮三扠神經電刺激%Morris水迷宮試驗
전간%필라잡품%경피삼차신경전자격%Morris수미궁시험
epilepsy%Pilocarpine%percutaneous trigeminal nerve electrical stimulation%Morris water maze test
目的 探讨经皮三叉神经电刺激对匹罗卡品诱导的癫痫大鼠的行为及学习记忆能力的影响.方法 健康雄性大鼠30只,随机平均分成正常对照组、癫痫模型组和经皮三叉神经电刺激组( TNS组). 采用匹罗卡品诱导癫痫持续状态制作大鼠模型. TNS组大鼠给予经皮三叉神经电刺激处理4周. 模型建立2周后,视频脑电监测大鼠自发性发作的频率、发作强度以及持续时间. 模型建立4周后,各组大鼠进行Morris水迷宫试验,记录大鼠在获得性训练中寻找水下平台的逃避潜伏期,以及在空间探索试验中搜索目标象限的时间百分比、航行距离百分比和穿越平台的次数. 结果 正常对照组大鼠在实验全程均未出现异常行为. 动物模型制作过程中,癫痫模型组有1只大鼠死亡,TNS组有2只大鼠死亡. 视频脑电监测期间,癫痫模型组有2只大鼠死亡. 与癫痫模型组比较,TNS组大鼠2周内自发性发作频率明显降低,发作持续时间明显缩短,发作级别评分明显降低( P<0.05~0.01). 在获得性训练中,随着训练天数的增加,各组大鼠平均逃避潜伏期逐渐缩短;与正常对照组比较,癫痫模型组大鼠各时间点平均逃避潜伏期均明显延长(均P<0.01 );与癫痫模型组比较, TNS组大鼠各时间点平均逃避潜伏期均明显缩短( P<0.05~0.01). 与正常对照组比较,癫痫模型组和TNS组大鼠在目标象限搜索的时间百分比及航行距离百分比明显降低,穿越平台的次数明显减少(均P<0.01);与癫痫模型组比较,TNS组大鼠在目标象限搜索的时间百分比及航行距离百分比显著增高,穿越平台的次数明显增多( P<0.05~0.01). 结论 经皮三叉神经电刺激可减少匹罗卡品诱导的癫痫大鼠的自发性发作,保护其学习记忆能力.
目的 探討經皮三扠神經電刺激對匹囉卡品誘導的癲癇大鼠的行為及學習記憶能力的影響.方法 健康雄性大鼠30隻,隨機平均分成正常對照組、癲癇模型組和經皮三扠神經電刺激組( TNS組). 採用匹囉卡品誘導癲癇持續狀態製作大鼠模型. TNS組大鼠給予經皮三扠神經電刺激處理4週. 模型建立2週後,視頻腦電鑑測大鼠自髮性髮作的頻率、髮作彊度以及持續時間. 模型建立4週後,各組大鼠進行Morris水迷宮試驗,記錄大鼠在穫得性訓練中尋找水下平檯的逃避潛伏期,以及在空間探索試驗中搜索目標象限的時間百分比、航行距離百分比和穿越平檯的次數. 結果 正常對照組大鼠在實驗全程均未齣現異常行為. 動物模型製作過程中,癲癇模型組有1隻大鼠死亡,TNS組有2隻大鼠死亡. 視頻腦電鑑測期間,癲癇模型組有2隻大鼠死亡. 與癲癇模型組比較,TNS組大鼠2週內自髮性髮作頻率明顯降低,髮作持續時間明顯縮短,髮作級彆評分明顯降低( P<0.05~0.01). 在穫得性訓練中,隨著訓練天數的增加,各組大鼠平均逃避潛伏期逐漸縮短;與正常對照組比較,癲癇模型組大鼠各時間點平均逃避潛伏期均明顯延長(均P<0.01 );與癲癇模型組比較, TNS組大鼠各時間點平均逃避潛伏期均明顯縮短( P<0.05~0.01). 與正常對照組比較,癲癇模型組和TNS組大鼠在目標象限搜索的時間百分比及航行距離百分比明顯降低,穿越平檯的次數明顯減少(均P<0.01);與癲癇模型組比較,TNS組大鼠在目標象限搜索的時間百分比及航行距離百分比顯著增高,穿越平檯的次數明顯增多( P<0.05~0.01). 結論 經皮三扠神經電刺激可減少匹囉卡品誘導的癲癇大鼠的自髮性髮作,保護其學習記憶能力.
목적 탐토경피삼차신경전자격대필라잡품유도적전간대서적행위급학습기억능력적영향.방법 건강웅성대서30지,수궤평균분성정상대조조、전간모형조화경피삼차신경전자격조( TNS조). 채용필라잡품유도전간지속상태제작대서모형. TNS조대서급여경피삼차신경전자격처리4주. 모형건립2주후,시빈뇌전감측대서자발성발작적빈솔、발작강도이급지속시간. 모형건립4주후,각조대서진행Morris수미궁시험,기록대서재획득성훈련중심조수하평태적도피잠복기,이급재공간탐색시험중수색목표상한적시간백분비、항행거리백분비화천월평태적차수. 결과 정상대조조대서재실험전정균미출현이상행위. 동물모형제작과정중,전간모형조유1지대서사망,TNS조유2지대서사망. 시빈뇌전감측기간,전간모형조유2지대서사망. 여전간모형조비교,TNS조대서2주내자발성발작빈솔명현강저,발작지속시간명현축단,발작급별평분명현강저( P<0.05~0.01). 재획득성훈련중,수착훈련천수적증가,각조대서평균도피잠복기축점축단;여정상대조조비교,전간모형조대서각시간점평균도피잠복기균명현연장(균P<0.01 );여전간모형조비교, TNS조대서각시간점평균도피잠복기균명현축단( P<0.05~0.01). 여정상대조조비교,전간모형조화TNS조대서재목표상한수색적시간백분비급항행거리백분비명현강저,천월평태적차수명현감소(균P<0.01);여전간모형조비교,TNS조대서재목표상한수색적시간백분비급항행거리백분비현저증고,천월평태적차수명현증다( P<0.05~0.01). 결론 경피삼차신경전자격가감소필라잡품유도적전간대서적자발성발작,보호기학습기억능력.
Objective To observe the effects of percutaneous trigeminal nerve electrical stimulation on behavior and the ability of learning and memory of epileptic rats induced by Pilocarpine.Methods Thirty healthy male rats were randomly divided into normal control group, epilepsy model group and trigeminal nerve stimulation group ( TNS group) .Pilocarpine was used to inducestatus epileptics ( SE) in rats.TNS group rats were treated with percutaneous trigeminal nerve stimulation consecutive for 4 weeks.At 2 weeks after SE model established, video monitor were employed to record the frequency of spontaneous recurrence seizures ( SRS) , the intensity and duration of seizures. At 4 weeks after SE model established, rats in each group were taken Morris water maze test.The incubation period of rats finding underwater platform in the acquired training was recorded.The percentage of time and distance of rats searching target quadrant and the number of times through the platform were also recorded.Results Rats in control group didn''t show abnormal behavior throughout this experiment.During the process of animal model making, a rat died in epilepsy model group while 2 in TNS group.During-EEG monitoring, 2 rats in epilepsy model group died. Compared with epilepsy model group, the frequency of spontaneous recurrence seizure and the intensity of seizure significantly decreased, the average time of seizure significantly shortened in TNS group ( P<0.05 -0.01 ) in 2 weeks.In acquired training experiment, with the number of training increased, the average escape latent of experimental animals gradually reduced, the escape latent of rats in epilepsy model group were significantly prolonged compared with control group rats at each time-point ( all P<0.01 ) , while the average escape latency was significantly reduced in TNS group rats than epilepsy model group (P<0.05-0.01).Compared to control group, the percentage of time search and the percentage of navigation distance in the target quadrant and the number of cross platform were significantly decreased in epilepsy model group and TNS group rats (all P<0.01).Compared to epilepsy model group, the percentage of time search and the percentage of navigation distance in the target quadrant within 60s were significantly higher and more cross platform numbers in TNS group ( P<0.05-0.01) .Conclusion In experimental epilepsy, percutaneous trigeminal nerve stimulation can reduce spontaneous recurrence seizures of the temporal lobe epilepsy rat and protect the ability of learning and memory.
