石油学报（石油加工）
石油學報（石油加工）
석유학보（석유가공）
Acta Petrolei Sinica (Petroleum Processing Section)
2015年
5期
1136-1143
,共8页
仲慧赟%刘芳%吕玉翠%陆津津%杨伟
仲慧赟%劉芳%呂玉翠%陸津津%楊偉
중혜빈%류방%려옥취%륙진진%양위
介孔SiO2 微球%固定化%溶菌酶%柴油泄漏%循环冷却水%缓蚀
介孔SiO2 微毬%固定化%溶菌酶%柴油洩漏%循環冷卻水%緩蝕
개공SiO2 미구%고정화%용균매%시유설루%순배냉각수%완식
mesoporous SiO2 microspheres%immobilization%lysozyme%diesel leakage%circulating cooling water%corrosion inhibition
采用溶胶‐凝胶法合成了介孔SiO2微球，并采用T EM、BET及FT‐IR谱对其进行表征。以介孔SiO2微球为载体，利用物理吸附法制备固定化溶菌酶，优化了制备条件，并将在优化条件下制备的固定化溶菌酶投加于柴油泄漏循环水中，考察其对碳钢的缓蚀效果。结果表明，制备固定化溶菌酶的最佳条件为溶菌酶质量浓度0.8 g／L、固定化时间10 h、pH值6.5、缓冲溶液摩尔浓度50 mmol／L。在柴油质量浓度为80 mg／L的冷却循环水中，固定化溶菌酶对碳钢的缓蚀率最高可达78.21％，且稳定性比游离溶菌酶有显著提高。
採用溶膠‐凝膠法閤成瞭介孔SiO2微毬，併採用T EM、BET及FT‐IR譜對其進行錶徵。以介孔SiO2微毬為載體，利用物理吸附法製備固定化溶菌酶，優化瞭製備條件，併將在優化條件下製備的固定化溶菌酶投加于柴油洩漏循環水中，攷察其對碳鋼的緩蝕效果。結果錶明，製備固定化溶菌酶的最佳條件為溶菌酶質量濃度0.8 g／L、固定化時間10 h、pH值6.5、緩遲溶液摩爾濃度50 mmol／L。在柴油質量濃度為80 mg／L的冷卻循環水中，固定化溶菌酶對碳鋼的緩蝕率最高可達78.21％，且穩定性比遊離溶菌酶有顯著提高。
채용용효‐응효법합성료개공SiO2미구，병채용T EM、BET급FT‐IR보대기진행표정。이개공SiO2미구위재체，이용물리흡부법제비고정화용균매，우화료제비조건，병장재우화조건하제비적고정화용균매투가우시유설루순배수중，고찰기대탄강적완식효과。결과표명，제비고정화용균매적최가조건위용균매질량농도0.8 g／L、고정화시간10 h、pH치6.5、완충용액마이농도50 mmol／L。재시유질량농도위80 mg／L적냉각순배수중，고정화용균매대탄강적완식솔최고가체78.21％，차은정성비유리용균매유현저제고。
Mesoporous SiO2 microspheres were synthesized by using sol‐gel method , and characterized by TEM ,BET and FT‐IR .Lysozyme was immobilized on mesoporous SiO2 through physical adsorption and the conditions of immobilization were optimized .The immobilized lysozyme prepared under optimal conditions was used as corrosion inhibitor in cooling circulating water with diesel leakage .The results showed that the optimal preparation conditions for immobilized lysozyme were lysozyme mass concentration of 0.8 g/L ,immobilization time of 10 h ,the pH value of 6.5 and the buffer molar concentration of 50 mmol/L .In the cooling circulating water containing diesel of 80 mg/L and with immobilized lysozyme as corrosion inhibitor ,the highest corrosion inhibition rate for carbon steel was 78.21% ,and the stability of immobilized lysozyme had increased obviously compared to free lysozyme .
