广东医学
廣東醫學
엄동의학
Guangdong Medical Journal
2015年
18期
2769-2772
,共4页
膀胱癌%NDPK-A%线粒体%超微结构%增殖抑制
膀胱癌%NDPK-A%線粒體%超微結構%增殖抑製
방광암%NDPK-A%선립체%초미결구%증식억제
bladder cancer%NDPK-A%mitochondria%ultrastructure%proliferation inhibition
目的 探讨NDPK-A过表达对人膀胱癌细胞株T24增殖能力和超微结构的影响. 方法 通过脂质体转染和G418筛选,建立稳定表达外源NDPK-A的T24细胞株,免疫细胞化学法检测细胞内NDPK-A表达, MTT法测定细胞增殖能力,透射电镜观察细胞的线粒体超微形态. 结果 NDPK-A过表达对T24细胞的增殖有抑制作用,引起了T24细胞内线粒体的固缩、内嵴变形,或发生肿胀和内部空洞化. 结论 NDPK-A对膀胱癌细胞T24的线粒体有破坏作用,可能通过抑制细胞的新陈代谢而抑制其增殖.
目的 探討NDPK-A過錶達對人膀胱癌細胞株T24增殖能力和超微結構的影響. 方法 通過脂質體轉染和G418篩選,建立穩定錶達外源NDPK-A的T24細胞株,免疫細胞化學法檢測細胞內NDPK-A錶達, MTT法測定細胞增殖能力,透射電鏡觀察細胞的線粒體超微形態. 結果 NDPK-A過錶達對T24細胞的增殖有抑製作用,引起瞭T24細胞內線粒體的固縮、內嵴變形,或髮生腫脹和內部空洞化. 結論 NDPK-A對膀胱癌細胞T24的線粒體有破壞作用,可能通過抑製細胞的新陳代謝而抑製其增殖.
목적 탐토NDPK-A과표체대인방광암세포주T24증식능력화초미결구적영향. 방법 통과지질체전염화G418사선,건립은정표체외원NDPK-A적T24세포주,면역세포화학법검측세포내NDPK-A표체, MTT법측정세포증식능력,투사전경관찰세포적선립체초미형태. 결과 NDPK-A과표체대T24세포적증식유억제작용,인기료T24세포내선립체적고축、내척변형,혹발생종창화내부공동화. 결론 NDPK-A대방광암세포T24적선립체유파배작용,가능통과억제세포적신진대사이억제기증식.
Objective To investigate the effect of NDPK-A overexpression on the proliferation and ultrastructure of the T24 human bladder cancer cell line.Methods T24 cells were transfected by NDPK-A expressing plasmid with the aid of lipofectamine, followed by screening under the pressure of G418.Expression of NDPK-A protein was detected by immunocytochemistry.Cell proliferation was determined by MTT assay, and ultrastructure of mitochondria was observed under transmission electron microscope.Results Overexpression of NDPK-A not only caused significant inhibition of proliferation in T24 cells, but also led to pycnosis, swelling, cristae deformation and internal cavitation in mitochondria of T24 cells.Conclusion NDPK-A has detrimental effects on mitochondria in the T24 bladder cancer cells.Inhibitory effect on mitochondria metabolism might be one of the mechanisms through which NDPK-A suppresses proliferation of T24 cells.
