广东医学
廣東醫學
엄동의학
Guangdong Medical Journal
2015年
18期
2783-2787
,共5页
脊髓损伤%人脐血CD34+细胞%组织活力%血管密度%神经分化
脊髓損傷%人臍血CD34+細胞%組織活力%血管密度%神經分化
척수손상%인제혈CD34+세포%조직활력%혈관밀도%신경분화
spinal cord injury%human umbilical cord blood CD34 +cells%tissue vitality%blood vessel density%neural differentiation
目的 探讨人脐血CD34 +细胞急性期移植修复脊髓损伤的效果和血管修复机制. 方法 免疫磁珠法从人新鲜脐血中分离得到CD34+细胞. 雌性Wistar大鼠96只,以IMPACTOR MODEL-Ⅱ脊髓损伤打击器建立T10脊髓损伤模型,随机平均分成A、B、C 3组:A组造模后给予PBS局部移植;B组造模后给予环孢霉素A灌胃联合地塞米松静脉注入治疗,C组造模后给予细胞局部移植及环孢霉素A灌胃联合地塞米松静脉注入治疗. 对各组后肢功能恢复情况进行BBB评分,损伤中心行双重免疫荧光染色、2,3,5-氯化三苯基四氮唑( TTC)染色和血管明胶墨汁灌注观察. 结果 各组大鼠脊髓损伤后后肢功能均逐渐改善,C组存活移植细胞虽未观察到神经分化,但BBB评分均高于其余两组(P<0.01),TTC染色显示组织活力降低区域比例小于其余两组(P<0.01),血管明胶墨汁灌注血管密度大于其余两组(P<0.01). 组织活力的恢复、损伤中心血管密度的增大和后肢功能的改善具有一致性变化. 结论 人脐血CD34 +细胞可能通过提高脊髓损伤中心血管密度促进微循环恢复,增加组织活力,促进大鼠脊髓损伤后肢体功能恢复.
目的 探討人臍血CD34 +細胞急性期移植脩複脊髓損傷的效果和血管脩複機製. 方法 免疫磁珠法從人新鮮臍血中分離得到CD34+細胞. 雌性Wistar大鼠96隻,以IMPACTOR MODEL-Ⅱ脊髓損傷打擊器建立T10脊髓損傷模型,隨機平均分成A、B、C 3組:A組造模後給予PBS跼部移植;B組造模後給予環孢黴素A灌胃聯閤地塞米鬆靜脈註入治療,C組造模後給予細胞跼部移植及環孢黴素A灌胃聯閤地塞米鬆靜脈註入治療. 對各組後肢功能恢複情況進行BBB評分,損傷中心行雙重免疫熒光染色、2,3,5-氯化三苯基四氮唑( TTC)染色和血管明膠墨汁灌註觀察. 結果 各組大鼠脊髓損傷後後肢功能均逐漸改善,C組存活移植細胞雖未觀察到神經分化,但BBB評分均高于其餘兩組(P<0.01),TTC染色顯示組織活力降低區域比例小于其餘兩組(P<0.01),血管明膠墨汁灌註血管密度大于其餘兩組(P<0.01). 組織活力的恢複、損傷中心血管密度的增大和後肢功能的改善具有一緻性變化. 結論 人臍血CD34 +細胞可能通過提高脊髓損傷中心血管密度促進微循環恢複,增加組織活力,促進大鼠脊髓損傷後肢體功能恢複.
목적 탐토인제혈CD34 +세포급성기이식수복척수손상적효과화혈관수복궤제. 방법 면역자주법종인신선제혈중분리득도CD34+세포. 자성Wistar대서96지,이IMPACTOR MODEL-Ⅱ척수손상타격기건립T10척수손상모형,수궤평균분성A、B、C 3조:A조조모후급여PBS국부이식;B조조모후급여배포매소A관위연합지새미송정맥주입치료,C조조모후급여세포국부이식급배포매소A관위연합지새미송정맥주입치료. 대각조후지공능회복정황진행BBB평분,손상중심행쌍중면역형광염색、2,3,5-록화삼분기사담서( TTC)염색화혈관명효묵즙관주관찰. 결과 각조대서척수손상후후지공능균축점개선,C조존활이식세포수미관찰도신경분화,단BBB평분균고우기여량조(P<0.01),TTC염색현시조직활력강저구역비례소우기여량조(P<0.01),혈관명효묵즙관주혈관밀도대우기여량조(P<0.01). 조직활력적회복、손상중심혈관밀도적증대화후지공능적개선구유일치성변화. 결론 인제혈CD34 +세포가능통과제고척수손상중심혈관밀도촉진미순배회복,증가조직활력,촉진대서척수손상후지체공능회복.
Objective To observe the efficacy of human umbilical cord blood CD34 +cells transplantation on spi-nal cord injury and to investigate the mechanism of vascular repair.Methods CD34 +cells were separated from fresh hu-man umbilical cord blood by magnetic-activated cell sorting.96 female Wistar rats were injured at T10 by IMPACTOR MODEL-Ⅱ, then randomly assigned to 3 groups:Group A, local injection of PBS into the injured epicenter of the spinal cord;Group B, intragastric administration of cyclosporin A and intravenous infusion of dexamethasone; Group C, local transplantation of cells into the injured epicenter of the spinal cord combined with intragastric administration of cyclosporin A and intravenous infusion of dexamethasone.Recovery of the lower limbs were assessed using BBB locomotor scoring sys-tem.The survival and neural differentiation of transplanted cells at the injury site were observed by double immunofluores-cence.Tissue vitality at the injury site was observed by 2,3,5 -triphenyl-2H-tetrazolium chloride staining and the blood vessel density at the injury site was observed by infusing mixture of Chinese ink and glutin followed by HE staining. Results BBB score increased gradually in all groups.Transplanted cells could survive, but neural differentiation was not observed.Nevertheless, BBB score was higher in Group C than in the other groups (P<0.01).Percentage of the area with decreased vitality in Group C was smaller than the other groups ( P<0.01) .The blood vessel density in Group C was higher than the other groups ( P<0.01) .Recovery of the lower limbs was consistent with recovery of tissue vitality and in-crease of the blood vessel density.Conclusion Human umbilical cord blood CD34 +cells can promote recovery of the lower limbs after spinal cord injury by repairing blood vessels and restoring microcirculation to increase tissue vitality at the injury site in rats.
