CAJ | 학술논문

目的：研究金线莲正丁醇提取物的降血糖作用及其机制。方法以健康 SD 大鼠12只为正常对照组,将糖尿病模型大鼠48只随机分为模型对照组和金线莲正丁醇提取物大、中、小剂量组,每组12只。金线莲正丁醇提取物大、中、小剂量组分别灌胃给予金线莲正丁醇提取物600,400,200 mg.kg－1,正常对照组和模型对照组给予等体积0.9％氯化钠溶液,均每天1次。给药3周后,测定大鼠体质量、血清葡萄糖含量和血清胰岛素水平。结果实验结束时,与正常对照组比较,模型对照组大鼠体质量明显减轻(P<0.01),血清葡萄糖明显升高(P<0.01),与模型对照组比较,金线莲正丁醇提取物大、中、小剂量组体质量差异无统计学意义(P>0.05),血清葡萄糖明显降低,差异有统计学意义(P<0.01或P<0.05),金线莲正丁醇提取物大剂量组血清胰岛素水平明显升高(P<0.05)。结论金线莲正丁醇提取物具有显著降血糖作用,大剂量金线莲正丁醇提取物能显著增加大鼠胰岛素水平,其降糖机制可能与促进β细胞再生有关。
목적：연구금선련정정순제취물적강혈당작용급기궤제。방법이건강 SD 대서12지위정상대조조,장당뇨병모형대서48지수궤분위모형대조조화금선련정정순제취물대、중、소제량조,매조12지。금선련정정순제취물대、중、소제량조분별관위급여금선련정정순제취물600,400,200 mg.kg－1,정상대조조화모형대조조급여등체적0.9％록화납용액,균매천1차。급약3주후,측정대서체질량、혈청포도당함량화혈청이도소수평。결과실험결속시,여정상대조조비교,모형대조조대서체질량명현감경(P<0.01),혈청포도당명현승고(P<0.01),여모형대조조비교,금선련정정순제취물대、중、소제량조체질량차이무통계학의의(P>0.05),혈청포도당명현강저,차이유통계학의의(P<0.01혹P<0.05),금선련정정순제취물대제량조혈청이도소수평명현승고(P<0.05)。결론금선련정정순제취물구유현저강혈당작용,대제량금선련정정순제취물능현저증가대서이도소수평,기강당궤제가능여촉진β세포재생유관。
Objective To study the hypoglycemic effect and mechanism of n￣butyl alcohol extraction from Anoectochilus roxburghii. Methods The total of 12 normal rats were used as normal control group, and 48 diabetic model rats were divided into model control group, and high dose, middle dose and low dose treatment groups with 12 rats in each group,three treatment groups received Anoectochilus roxburghii at dose 600,400,200 mg.kg-1 , respectively, normal control group and model control group were administered with equal volume of 0.9% sodium chlorade solution once a day.After 21 days of drug administration, the weights of rats, the levels of blood glucose and insulin were examined. Results Compared with normal control group, the body weight in model control group decreased significantly(P<0.01), while blood glucose significantly increased (P<0.01).Compared with model control group, the body weight in Anoectochilus roxburghii groups exhibited no obvious difference,while blood glucose decreased significantly (P<0.01 or P<0.05).The level of insulin in high dose group increased remarkably (P<0.05). Conclusion The n￣butyl alcohol extraction from Anoectochilus roxburghii shows markedly hypoglycemic effect,specifically for which at high dose increases the level of insulin significantly,its mechanism may involve with promoting β cells regeneration.