CAJ | 학술논문

目的：探讨弗林蛋白酶(Furin)抑制剂在乳腺癌细胞增殖中的作用,为深入研究乳腺癌发生、发展机制提供理论基础。方法以不同浓度 Furin 抑制剂处理 MCF￣7细胞,噻唑蓝(MTT)法检测 Furin 抑制剂对 MCF￣7细胞增殖的影响,Hochest 33342染色法检测细胞凋亡,Western blot 检测细胞凋亡通路相关蛋白(Caspase￣3,Caspase￣8,Caspase￣9)表达水平,酶联免疫吸附(ELISA)法检测 Furin 抑制剂对细胞超氧化物调节相关酶类活性。结果 Furin 抑制剂对 MCF￣7细胞生长有明显抑制作用,而且抑制作用呈剂量、时间依赖性。 Hochest 33342染色发现 MCF￣7细胞出现明显凋亡；Western blot 结果显示,细胞凋亡通路相关蛋白 Caspase￣3,Caspase￣8及 Caspase￣9表达显著增高。一定浓度的 Furin 抑制剂作用细胞48 h 后,显著增高细胞内超氧化物歧化酶及过氧化氢酶活性。结论 Furin 抑制剂通过调节 MCF￣7细胞氧化还原状态,诱导 MCF￣7细胞凋亡,进而抑制细胞的增殖。
목적：탐토불림단백매(Furin)억제제재유선암세포증식중적작용,위심입연구유선암발생、발전궤제제공이론기출。방법이불동농도 Furin 억제제처리 MCF￣7세포,새서람(MTT)법검측 Furin 억제제대 MCF￣7세포증식적영향,Hochest 33342염색법검측세포조망,Western blot 검측세포조망통로상관단백(Caspase￣3,Caspase￣8,Caspase￣9)표체수평,매련면역흡부(ELISA)법검측 Furin 억제제대세포초양화물조절상관매류활성。결과 Furin 억제제대 MCF￣7세포생장유명현억제작용,이차억제작용정제량、시간의뢰성。 Hochest 33342염색발현 MCF￣7세포출현명현조망；Western blot 결과현시,세포조망통로상관단백 Caspase￣3,Caspase￣8급 Caspase￣9표체현저증고。일정농도적 Furin 억제제작용세포48 h 후,현저증고세포내초양화물기화매급과양화경매활성。결론 Furin 억제제통과조절 MCF￣7세포양화환원상태,유도 MCF￣7세포조망,진이억제세포적증식。
Objective To investigate the role of Furin in breast cancer cell proliferation and provide a theoretical basis for in￣depth study of breast cancer. Methods Different concentrations of Furin inhibitor were added in MCF￣7 cell culture to test MCF￣7 cell proliferation by MTT essay.Hochest 33342 staining was used to detect the morphological change of apoptosic cells.Western blot analysis was applied to measure the level of cell apoptosis associated proteins,such as Caspase￣3,Caspase￣8 andCaspase￣9.The enzyme￣linked immunosorbent assay was used for detection the CAT and SOD levels in cell culture. ResultsMCF￣7 cell growth was inhibited by Furin inhibitor in a time and dose dependent manner.The results of Western blot and Hochest33342 staining indicated that MCF￣7 cells were apoptosis after Furin inhibitor treatment. The level of CAT was increasedsignificantly,associated with the level of SOD. Conclusion Furin inhibitor could induce MCF cell apoptosis, thereby inhibitcell proliferation by modulating MCF￣7 cell redox state.