CAJ | 학술논문

目的：采用高效液相色谱法建立不同产地杜仲药材水提液指纹图谱。方法采用 SinoChrom ODS￣BP (250 mm×4.6 mm,5μm)色谱柱,乙腈￣0.1％磷酸溶液为流动相进行梯度洗脱,流速为1.0 mL.min－1,检测波长为230 nm,柱温25℃。结果确定14个共有峰,10批不同产地杜仲药材水提液的色谱指纹图谱整体相似度在0.592~0.986,并得出对照指纹图谱。结论该指纹图谱方法简便、重复性好,为有效地控制杜仲药材的内在质量提供科学依据。
목적：채용고효액상색보법건립불동산지두중약재수제액지문도보。방법채용 SinoChrom ODS￣BP (250 mm×4.6 mm,5μm)색보주,을정￣0.1％린산용액위류동상진행제도세탈,류속위1.0 mL.min－1,검측파장위230 nm,주온25℃。결과학정14개공유봉,10비불동산지두중약재수제액적색보지문도보정체상사도재0.592~0.986,병득출대조지문도보。결론해지문도보방법간편、중복성호,위유효지공제두중약재적내재질량제공과학의거。
Objective To establish fingerprint and mutual mode of aqueous extracts of Eucommia ulmoides Oliv.from different regions by high performance liquid chromatography ( HPLC). Methods The column of SinoChrom ODS￣BP (250 mm×4.6 mm, 5 μm) was used.The mobile phase consisted of acetonitrile￣0.1% phosphoric acid with gradient elution.The flow rate was 1.0 mL.min-1 , the detective wavelength was 230 nm, and the column temperature was 25 ℃ . Results The fingerprint consisted of 14 common peaks.The overall similarity for ten batches of Eucommia ulmoides Oliv.from different regions was 0.592-0.986.The standard fingerprint of Eucommia ulmoides Oliv.was established by HPLC. Conclusion This fingerprint method is simple and repeatable, and it provides a scientific basis for the quality control of Eucommia ulmoides Oliv.