医药导报
醫藥導報
의약도보
Herald of Medicine
2015年
11期
1483-1485
,共3页
叶勇%黄秋洁%刘华钢%陈光强%农玉红%杨丽婷%钟锦华
葉勇%黃鞦潔%劉華鋼%陳光彊%農玉紅%楊麗婷%鐘錦華
협용%황추길%류화강%진광강%농옥홍%양려정%종금화
战骨%柚皮素%芹菜素%色谱法,高效液相%含量测定
戰骨%柚皮素%芹菜素%色譜法,高效液相%含量測定
전골%유피소%근채소%색보법,고효액상%함량측정
Premna fulva Craib.%Naringenin%Apigenin%Chromatography,high performance liquid%Content determination
目的:建立测定战骨中柚皮素和芹菜素含量的高效液相色谱法。方法以 SHISEIDO ̄SPOLAR C18柱(250 mm×4.6 mm,5μm)为色谱柱,流动相采用甲醇 ̄0.2%磷酸梯度洗脱(42:58),柚皮素的检测波长为288 nm,芹菜素的检测波长为340 nm,流速为1.0 mL.min-1,柱温为35℃,进样量10μL。结果柚皮素在0.180~3.60μg 之间线性关系良好,r=0.9999,芹菜素在0.0052~0.1040μg 之间线性关系良好,r=0.9999。结论该方法准确、可靠,可用于战骨药材及相关制剂中柚皮素和芹菜素的含量测定。
目的:建立測定戰骨中柚皮素和芹菜素含量的高效液相色譜法。方法以 SHISEIDO ̄SPOLAR C18柱(250 mm×4.6 mm,5μm)為色譜柱,流動相採用甲醇 ̄0.2%燐痠梯度洗脫(42:58),柚皮素的檢測波長為288 nm,芹菜素的檢測波長為340 nm,流速為1.0 mL.min-1,柱溫為35℃,進樣量10μL。結果柚皮素在0.180~3.60μg 之間線性關繫良好,r=0.9999,芹菜素在0.0052~0.1040μg 之間線性關繫良好,r=0.9999。結論該方法準確、可靠,可用于戰骨藥材及相關製劑中柚皮素和芹菜素的含量測定。
목적:건립측정전골중유피소화근채소함량적고효액상색보법。방법이 SHISEIDO ̄SPOLAR C18주(250 mm×4.6 mm,5μm)위색보주,류동상채용갑순 ̄0.2%린산제도세탈(42:58),유피소적검측파장위288 nm,근채소적검측파장위340 nm,류속위1.0 mL.min-1,주온위35℃,진양량10μL。결과유피소재0.180~3.60μg 지간선성관계량호,r=0.9999,근채소재0.0052~0.1040μg 지간선성관계량호,r=0.9999。결론해방법준학、가고,가용우전골약재급상관제제중유피소화근채소적함량측정。
Objective To establish a HPLC method for determination of naringenin and apigenin in Premna fulva. Methods The SHISEIDO ̄SPOLAR C18(250 mm×4.6 mm,5μm) was used as analytical column.The mobile phase consisted of methanol ̄0.2% phosphoric acid (42:58) with isocratic elution at a flow rate of 1.0 mL.min-1 .The detection wavelength of naringenin and apigenin was 288 nm and 340 nm, respectively.Column temperature was set at 35 ℃ , the injection volumn was 10 μL. Results Naringenin and apigenin had a good linear relationship in the concentration range of 0.180 ~ 3.60 μg (r =0.999 9) and 0. 0052 ~ 0. 1040 μg ( r = 0. 999 9), respectively. Conclusion The method is accurate and reliable. It is appropriate for the quantitative determination of naringenin and apigenin in Premna fulva and its preparations.