中国中医药信息杂志
中國中醫藥信息雜誌
중국중의약신식잡지
Chinese Journal of Information on Traditional Chinese Medicine
2015年
11期
46-49
,共4页
贾歌刘畅%庞晶瑶%柏兆方%牛明%赵艳玲%朱云%张雅铭%马致洁%赵奎君
賈歌劉暢%龐晶瑤%柏兆方%牛明%趙豔玲%硃雲%張雅銘%馬緻潔%趙奎君
가가류창%방정요%백조방%우명%조염령%주운%장아명%마치길%조규군
何首乌%大黄素%大黄酸%没食子酸%肝细胞%凋亡%细胞周期
何首烏%大黃素%大黃痠%沒食子痠%肝細胞%凋亡%細胞週期
하수오%대황소%대황산%몰식자산%간세포%조망%세포주기
Polygoni Multiflori Radix%emodin%rhein%gallic acid%hepatocyte%apoptosis%cell cycle
目的 观察何首乌对人正常肝细胞的毒性和细胞凋亡的影响,探讨何首乌致肝损伤的可能物质和途径.方法 以人正常肝(实质)细胞系L02为评价模型,Giemsa染色观察给药后各组细胞形态,流式细胞术检测给药后各组细胞凋亡率及细胞周期.结果 与空白对照组比较,何首乌70%乙醇提取物1、2、4 mg原药材/mL对肝细胞形态和细胞周期影响均不明显,给药24 h凋亡率分别为9.51%、10.3%、13.2%;何首乌中3个成分大黄素、大黄酸和没食子酸(25、50、100 μmol/L)单独给药,各给药组细胞均可见典型凋亡形态,G1 期细胞所占比例明显减小,S期、G2期比例增加,其中3个成分高浓度(100μmol/L)给药24 h凋亡率分别为19.8%、12.0%和13.7%.结论 何首乌对人L02细胞有一定的诱导凋亡作用,其所含大黄素诱导凋亡作用强于大黄酸和没食子酸.
目的 觀察何首烏對人正常肝細胞的毒性和細胞凋亡的影響,探討何首烏緻肝損傷的可能物質和途徑.方法 以人正常肝(實質)細胞繫L02為評價模型,Giemsa染色觀察給藥後各組細胞形態,流式細胞術檢測給藥後各組細胞凋亡率及細胞週期.結果 與空白對照組比較,何首烏70%乙醇提取物1、2、4 mg原藥材/mL對肝細胞形態和細胞週期影響均不明顯,給藥24 h凋亡率分彆為9.51%、10.3%、13.2%;何首烏中3箇成分大黃素、大黃痠和沒食子痠(25、50、100 μmol/L)單獨給藥,各給藥組細胞均可見典型凋亡形態,G1 期細胞所佔比例明顯減小,S期、G2期比例增加,其中3箇成分高濃度(100μmol/L)給藥24 h凋亡率分彆為19.8%、12.0%和13.7%.結論 何首烏對人L02細胞有一定的誘導凋亡作用,其所含大黃素誘導凋亡作用彊于大黃痠和沒食子痠.
목적 관찰하수오대인정상간세포적독성화세포조망적영향,탐토하수오치간손상적가능물질화도경.방법 이인정상간(실질)세포계L02위평개모형,Giemsa염색관찰급약후각조세포형태,류식세포술검측급약후각조세포조망솔급세포주기.결과 여공백대조조비교,하수오70%을순제취물1、2、4 mg원약재/mL대간세포형태화세포주기영향균불명현,급약24 h조망솔분별위9.51%、10.3%、13.2%;하수오중3개성분대황소、대황산화몰식자산(25、50、100 μmol/L)단독급약,각급약조세포균가견전형조망형태,G1 기세포소점비례명현감소,S기、G2기비례증가,기중3개성분고농도(100μmol/L)급약24 h조망솔분별위19.8%、12.0%화13.7%.결론 하수오대인L02세포유일정적유도조망작용,기소함대황소유도조망작용강우대황산화몰식자산.
Objective To observe the effects of Polygoni Multiflori Radix on toxicity and apoptosis of human normal hepatocyte;To discuss the possible substances and channels of Polygoni Multiflori Radix to hepatic injury.Methods L02 cells were set as evaluation model. Cellular morphology in each group after medication was observed through Giemsa dyeing. Apoptosis rate and cell cycle of each group after medication were detected by FCM.Results Compared with blank control group, the 70% alcohol extracts of Polygoni Multiflori Radix (1, 2 and 4 mg raw materials per mL) had almost no effects on cellular morphology or cell cycle of L02 cells. Apoptosis rates after treatment with extracts of Polygoni Multiflori Radix were 9.51%, 10.3%, and 13.2% respectively. When L02 cells were treated with different dosages of emodin, rhein and gallic acid, three ingredients of Polygoni Multiflori Radix, apoptosis was obvious. The proportion of G1 phase cells significantly decreased, the S phase and G2 phase increased, which presented the concentration dependent effect. Apoptosis rates of three high dosages (100μmol/L) treated groups after treatment with emodin, rhein and gallic acid were 19.8%, 12.0%, and 13.7% respectively. Conclusion Polygoni Multiflori Radix has apoptosis-inducing effects on L02 cells. Apoptosis-inducing effects of its ingredient of emodin is stronger than rhein and gallic acid.
