CAJ | 학술논문

目的观察加味丹参饮对ApoE-/-动脉粥样硬化(AS)模型小鼠血清一氧化氮(NO)和内皮素(ET)及内皮细胞超微结构的影响,探讨其抗AS作用机制.方法 24只7~8周龄ApoE-/-小鼠饲以高脂饲料12周造模,随机分为模型组、阳性药组和加味丹参饮低、高剂量组,每组6只;同品系C57BL/6J小鼠作为空白组,饲以普通饲料.药物干预8周后,摘眼球取血测NO、ET水平,透射电镜观察主动脉内皮细胞超微结构变化.结果与空白组比较,模型组血清NO含量明显降低、ET含量明显升高(P<0.01);与模型组比较,各给药组NO含量明显升高、ET含量明显降低(P<0.05,P<0.01).电镜结果显示,模型组呈AS脂纹期表现,各给药组内皮损伤较模型组均有改善.结论加味丹参饮可改善内皮细胞超微结构、功能,保护内皮细胞,延缓AS进程.
목적 관찰가미단삼음대ApoE-/-동맥죽양경화(AS)모형소서혈청일양화담(NO)화내피소(ET)급내피세포초미결구적영향,탐토기항AS작용궤제.방법 24지7~8주령ApoE-/-소서사이고지사료12주조모,수궤분위모형조、양성약조화가미단삼음저、고제량조,매조6지;동품계C57BL/6J소서작위공백조,사이보통사료.약물간예8주후,적안구취혈측NO、ET수평,투사전경관찰주동맥내피세포초미결구변화.결과 여공백조비교,모형조혈청NO함량명현강저、ET함량명현승고(P<0.01);여모형조비교,각급약조NO함량명현승고、ET함량명현강저(P<0.05,P<0.01).전경결과현시,모형조정AS지문기표현,각급약조내피손상교모형조균유개선.결론 가미단삼음가개선내피세포초미결구、공능,보호내피세포,연완AS진정.
Objective To investigate the effects of Jiawei Danshen Decoction on endothelial cell ultrastructure, serum NO and ET in apolipoprotein E-knockout atherosclerosis (AS) mice;To discuss its mechanism of anti-atherosclerosis.Methods Totally 24 7-8 weeks old ApoE -/- mice were fed with a high fat diet for 12 weeks until the a mature atherosclerotic plaque was formed. They were randomly divided into four groups:model group, control group,Jiawei Danshen Decoction of low-dose group and high-dose group, 6 mice in each group. Another 6 normal C57BL/6J mice with the same strain were set as the blank group, and fed with general diet. After medicine intervention by gavage for 8 weeks, blood was extracted from orbital venous to measure serum NO and ET;aortic endothelial cell ultrastructure changes were observed by transmission electron microscope.Results Compared with the blank group, the level of NO obviously decreased and ET significantly increased in the model group (P<0.01). Compared with model group, medicine intervention increased NO and reduce ET level (P<0.05). Electron microscopy results showed that the model group showed fatty streaks stage performance;endothelial damage in each administration group was improved compared with model group.Conclusion Jiawei Danshen Decoction can improve the endothelial cell ultrastructure and functions and protect endothelial cells, with a purpose to delay the AS process.