CAJ | 학술논문

目的观察脊髓康对脊髓损伤大鼠血清脑源性神经营养因子(BDNF)及神经生长因子(NGF)蛋白表达的影响,探讨其改善损伤轴突再生微环境和抗脊髓损伤的作用机制.方法 90只SD大鼠采用改良Allen's法建立大鼠急性脊髓损伤模型,随机抽取15只作为假手术组,其余75只造模成功后随机分为模型组、强的松组及脊髓康高、中、低剂量组.各治疗组予相应药物灌胃,假手术组和模型组予等体积生理盐水灌胃.记录期间各组大鼠活动及死亡情况,于干预后3、7、14 d处死大鼠,颈动脉采血,ELISA检测血清BDNF、NGF表达.结果模型组大鼠脊髓损伤后均出现典型的截瘫综合征.术后3 d,BDNF表达模型组与假手术组比较差异无统计学意义(P>0.05),而各治疗组均高于假手术组(P<0.05,P<0.01);术后7、14 d,各治疗组和模型组均高于假手术组(P<0.01),其中强的松组、脊髓康中剂量组高于模型组(P<0.05,P<0.01).各治疗组和模型组NGF表达均高于假手术组,术后3、7 d各治疗组明显高于模型组;术后14 d与模型组比较,强的松组和脊髓康中、低剂量组仍维持较高水平(P<0.05,P<0.01).结论脊髓康能有效促进脊髓损伤大鼠血清 BDNF、NGF 表达,并在14 d内维持较高水平,从而改善轴突再生微环境,促进神经再生.
목적 관찰척수강대척수손상대서혈청뇌원성신경영양인자(BDNF)급신경생장인자(NGF)단백표체적영향,탐토기개선손상축돌재생미배경화항척수손상적작용궤제.방법 90지SD대서채용개량Allen's법건립대서급성척수손상모형,수궤추취15지작위가수술조,기여75지조모성공후수궤분위모형조、강적송조급척수강고、중、저제량조.각치료조여상응약물관위,가수술조화모형조여등체적생리염수관위.기록기간각조대서활동급사망정황,우간예후3、7、14 d처사대서,경동맥채혈,ELISA검측혈청BDNF、NGF표체.결과 모형조대서척수손상후균출현전형적절탄종합정.술후3 d,BDNF표체모형조여가수술조비교차이무통계학의의(P>0.05),이각치료조균고우가수술조(P<0.05,P<0.01);술후7、14 d,각치료조화모형조균고우가수술조(P<0.01),기중강적송조、척수강중제량조고우모형조(P<0.05,P<0.01).각치료조화모형조NGF표체균고우가수술조,술후3、7 d각치료조명현고우모형조;술후14 d여모형조비교,강적송조화척수강중、저제량조잉유지교고수평(P<0.05,P<0.01).결론 척수강능유효촉진척수손상대서혈청 BDNF、NGF 표체,병재14 d내유지교고수평,종이개선축돌재생미배경,촉진신경재생.
Objective To clarify the effects of Jisuikang on expression levels of BDNF and NGF protein in serum of rats with spinal cord injury;To explore its probable mechanism to improve the axon regeneration microenvironment and resistance spinal cord injury.Methods Ninety SD rats were chosen to establish acute spinal cord injury model through modified Allen's method. 15 rats were chosen randomly as sham-operation group, and the other 75 rats were randomly divided into model group, prednisone group and high-, medium-, and low-doseJisuikang groups. All administration groups were given relevant medicine for gavage, while rats in sham-operation group and model group were given normal saline with the same volume for gavage. The activity and death condition of rats were recorded. Rats were put to death on the 3rd, 7th and 14th d of intervention for carotid artery blood collection. The expressions of BDNF and NGF in serum were detected by ELISA.Results After modeling, the rats in model group showed a typical paraplegia syndrome. 3 day after operation, BDNF showed no statistical significance between model group and prednisone group, while each treatment group showed significant difference when compared with prednisone group (P<0.05,P<0.01). 7 d and 14 d after operation, compared with the model group, BDNF of prednisone group and medium-dose Jisuikang group showed statistical significance, and there was no significant difference between prednisone group and medium-dose Jisuikang group (P<0.05,P<0.01). The expression of NGF in each treatment group at each time point with the prednisone group showed statistical significance. 3 d and 7 d after operation, among prednisone group, high- and medium-doseJisuikang groups, the expression of NGF was significantly higher than model group. 14 d after operation, compared with the model group, the expression of NGF in medium- and low-dose Jisuikang groups and prednisone group still maintained at a relatively high level, with statistical significance (P<0.05,P<0.01).Conclusion Jisuikang can effectively promote the expressions of BDNF and NGF in serum after spinal cord injury and maintained at a relatively high level, so as to improve the microenvironment of axonal regeneration and promote nerve regeneration.