中国中医药信息杂志
中國中醫藥信息雜誌
중국중의약신식잡지
Chinese Journal of Information on Traditional Chinese Medicine
2015年
11期
73-75
,共3页
白疕合剂%人永生化角质形成细胞%血管内皮生长因子%血管内皮生长因子受体-2%大鼠
白疕閤劑%人永生化角質形成細胞%血管內皮生長因子%血管內皮生長因子受體-2%大鼠
백비합제%인영생화각질형성세포%혈관내피생장인자%혈관내피생장인자수체-2%대서
White Mange Mixture%HaCaT cells%VEGF%VEGFR-2%rats
目的 观察中药白疕合剂干预人永生化角质形成细胞(HaCaT 细胞)后,对其血管内皮生长因子(VEGF)的分泌量及其受体2(VEGFR-2)基因和蛋白表达的影响,并探讨其作用机制.方法 体外培养HaCaT细胞建立银屑病实验模型.SD大鼠随机分为空白血清组、阿维A组和白疕合剂低、中、高剂量组,并单设空白对照组.给药后制备血清,干预HaCaT细胞模型.ELISA检测VEGF分泌量,RT-PCR检测VEGFR-2基因表达,Western blot检测VEGFR-2蛋白表达.结果 与空白对照组比较,白疕合剂低、中、高剂量组干预HaCaT细胞后,VEGF分泌量及VEGFR-2基因和蛋白表达均明显抑制,且呈浓度依赖性.结论 白疕合剂可能通过抑制VEGF分泌量、降低VEGFR-2基因和蛋白表达以达到治疗银屑病的作用.
目的 觀察中藥白疕閤劑榦預人永生化角質形成細胞(HaCaT 細胞)後,對其血管內皮生長因子(VEGF)的分泌量及其受體2(VEGFR-2)基因和蛋白錶達的影響,併探討其作用機製.方法 體外培養HaCaT細胞建立銀屑病實驗模型.SD大鼠隨機分為空白血清組、阿維A組和白疕閤劑低、中、高劑量組,併單設空白對照組.給藥後製備血清,榦預HaCaT細胞模型.ELISA檢測VEGF分泌量,RT-PCR檢測VEGFR-2基因錶達,Western blot檢測VEGFR-2蛋白錶達.結果 與空白對照組比較,白疕閤劑低、中、高劑量組榦預HaCaT細胞後,VEGF分泌量及VEGFR-2基因和蛋白錶達均明顯抑製,且呈濃度依賴性.結論 白疕閤劑可能通過抑製VEGF分泌量、降低VEGFR-2基因和蛋白錶達以達到治療銀屑病的作用.
목적 관찰중약백비합제간예인영생화각질형성세포(HaCaT 세포)후,대기혈관내피생장인자(VEGF)적분비량급기수체2(VEGFR-2)기인화단백표체적영향,병탐토기작용궤제.방법 체외배양HaCaT세포건립은설병실험모형.SD대서수궤분위공백혈청조、아유A조화백비합제저、중、고제량조,병단설공백대조조.급약후제비혈청,간예HaCaT세포모형.ELISA검측VEGF분비량,RT-PCR검측VEGFR-2기인표체,Western blot검측VEGFR-2단백표체.결과 여공백대조조비교,백비합제저、중、고제량조간예HaCaT세포후,VEGF분비량급VEGFR-2기인화단백표체균명현억제,차정농도의뢰성.결론 백비합제가능통과억제VEGF분비량、강저VEGFR-2기인화단백표체이체도치료은설병적작용.
Objective To observe the effects of White Mange Mixture on the content of VEGF and the genetic and protein expressions of VEGFR-2 of HaCaT cells;To explore its mechanism. Methods HaCaT cells were cultured in vitro to establish psoriasis experimental model. SD rats were randomly divided into the blank serum group, white mange mixture of low-, medium- and high- dose groups and acitretin group, meanwhile to set up blank control group. Cultured HaCaT cells model were intervened with different medicine serum. ELISA was applied to detect the content of VEGF. RT-PCR and Western blot were used to detect the genetic and protein expressions of VEGFR-2.Results Compared with the blank control group, White Mange Mixture of low-, medium- and high-dose groups interventing HaCaT cells could obviously inhibit the secretion of VEGF and decrease the genetic and protein expression of VEGFR-2 in a dose-dependent manner. Conclusion White Mange Mixture plays a role in the treatment of psoriasis possibly through inhibiting the content of VEGF and decreasing the genetic and protein expressions of VEGFR-2.
