CAJ | 학술논문

目的：探讨锰超氧化物歧化酶（MnSOD）基因 rs4880位点多态性与2型糖尿病（T2DM）发生及认知功能的相关性。方法选取2008年3月—2010年3月唐山工人医院住院治疗的 T2DM 患者450例为病例组，同期于北京某社区招募无 T2DM 者512例为对照组。检测受试者空腹血糖（FPG）及三酰甘油（TG）、总胆固醇（TC）、高密度脂蛋白胆固醇（HDL - C）、低密度脂蛋白胆固醇（ LDL - C）临床表型。采用重复性成套神经心理状态测验量表（RBANS）评价受试者认知功能。采用限制性片段长度多态性 PCR 技术检测 MnSOD 基因 rs4880位点多态性。结果MnSOD 基因 rs4880位点基因型为 TT、CT 和 CC。两组 rs4880位点基因型及等位基因频率分布比较，差异均无统计学意义（P ﹥0.05）。两组女性受试者 rs4880位点基因型分布比较，差异有统计学意义（P ﹤0.05）；其中，CT 基因型受试者发生 T2DM 的风险是 TT 基因型的2.188倍〔95％ CI（1.413，3.388）〕。两组女性受试者 rs4880位点等位基因频率分布比较，差异有统计学意义（P ﹤0.05）；其中，C 等位基因发生 T2DM 的风险是 T 等位基因的1.926倍〔95％ CI （1.310，2.830）〕。两组男性受试者 rs4880位点基因型及等位基因频率分布比较，差异均无统计学意义（ P ﹥0.05）。病例组 rs4880位点多态性与 TC、LDL - C 及胰岛素抵抗指数（HOMA - IR）相关（P ﹤0.05），而未发现与 FPG、TG、HDL - C 相关（P ﹥0.05）。病例组 rs4880位点多态性与 RBANS 延迟记忆相关（P ﹤0.05），而未发现与即刻记忆、视觉广度、言语功能、注意及总分相关（P ﹥0.05）。结论 MnSOD 基因 rs4880位点 CT、C 是女性 T2DM 发病的危险基因型和等位基因，T2DM 患者 RBANS 延迟记忆评分与 MnSOD 基因 rs4880位点多态性有关。
목적：탐토맹초양화물기화매（MnSOD）기인 rs4880위점다태성여2형당뇨병（T2DM）발생급인지공능적상관성。방법선취2008년3월—2010년3월당산공인의원주원치료적 T2DM 환자450례위병례조，동기우북경모사구초모무 T2DM 자512례위대조조。검측수시자공복혈당（FPG）급삼선감유（TG）、총담고순（TC）、고밀도지단백담고순（HDL - C）、저밀도지단백담고순（ LDL - C）림상표형。채용중복성성투신경심리상태측험량표（RBANS）평개수시자인지공능。채용한제성편단장도다태성 PCR 기술검측 MnSOD 기인 rs4880위점다태성。결과MnSOD 기인 rs4880위점기인형위 TT、CT 화 CC。량조 rs4880위점기인형급등위기인빈솔분포비교，차이균무통계학의의（P ﹥0.05）。량조녀성수시자 rs4880위점기인형분포비교，차이유통계학의의（P ﹤0.05）；기중，CT 기인형수시자발생 T2DM 적풍험시 TT 기인형적2.188배〔95％ CI（1.413，3.388）〕。량조녀성수시자 rs4880위점등위기인빈솔분포비교，차이유통계학의의（P ﹤0.05）；기중，C 등위기인발생 T2DM 적풍험시 T 등위기인적1.926배〔95％ CI （1.310，2.830）〕。량조남성수시자 rs4880위점기인형급등위기인빈솔분포비교，차이균무통계학의의（ P ﹥0.05）。병례조 rs4880위점다태성여 TC、LDL - C 급이도소저항지수（HOMA - IR）상관（P ﹤0.05），이미발현여 FPG、TG、HDL - C 상관（P ﹥0.05）。병례조 rs4880위점다태성여 RBANS 연지기억상관（P ﹤0.05），이미발현여즉각기억、시각엄도、언어공능、주의급총분상관（P ﹥0.05）。결론 MnSOD 기인 rs4880위점 CT、C 시녀성 T2DM 발병적위험기인형화등위기인，T2DM 환자 RBANS 연지기억평분여 MnSOD 기인 rs4880위점다태성유관。
Objective To investigate the relationship between Manganese superoxide dismutase(MnSOD) - rs4880 polymorphism and the development of type 2 diabetes(T2DM)as well as cognitive function. Methods We enrolled 450 T2DM patients who were hospitalized in Tangshan Gongren Hospital from March 2008 to March 2010 as the case group,and we enrolled another 512 persons without T2DM that were recruited from a community in Beijing in the same period as the control group. We collected the clinical phenotype data including FPG,TG,TC,HDL - C and LDL - C. The Repeatable Battery for the Assessment of Neuropsychological Status(RBANS)was applied to assess the cognitive function of the subjects,and RFLP - PCR was used to detect MnSOD - rs4880 polymorphism. Results MnSOD - rs4880 has three genotypes:TT,CT and CC. There was no significant difference both in distribution of genotype and allele frequency between the two groups(P ﹥ 0. 05). Significant difference was found in genotype distribution of this site in female subjects between the two groups(P ﹤ 0. 05);subjects with CT genotype had 2. 188 times higher risk to develop T2DM than TT genotype carriers〔95% CI(1. 413,3. 388)〕. Significant difference was also found in allele frequency of rs4880 site in female subjects between the two groups(P ﹤ 0. 05);subjects who carried C allele had 1. 926 times higher risk to develop T2DM than T allele carriers〔95% CI(1. 310,2. 830)〕. There was no significant difference both in the distribution of genotype and allele frequency of rs4880 site in male subjects between the two groups(P ﹥ 0. 05). Association was found between rs4880 site polymorphism and TC,LDL - C and HOMA - IR(P ﹤ 0. 05), while no association was found between rs4880 site polymorphism and FPG,TG and HDL - C( P ﹥ 0. 05). The rs4880 site polymorphism was found associated with RBANS delayed memory(P ﹤ 0. 05),while no association was found between MnSOD- rs4880 polymorphism and immediate memory,visuospatial/ constructional,language,attention and the total score( P ﹥0. 05). Conclusion CT and C are risk genotype and allele for females to develop T2DM,and MnSOD - rs4880 polymorphism is associated with RBANS delayed memory score of T2DM patients.