中国中医药信息杂志
中國中醫藥信息雜誌
중국중의약신식잡지
Chinese Journal of Information on Traditional Chinese Medicine
2015年
11期
92-94
,共3页
周文杰%陈乾平%曾友保%王桂秀
週文傑%陳乾平%曾友保%王桂秀
주문걸%진건평%증우보%왕계수
五指那藤%齐墩果酸%含量测定%反相高效液相色谱法
五指那籐%齊墩果痠%含量測定%反相高效液相色譜法
오지나등%제돈과산%함량측정%반상고효액상색보법
Stauntonia obovatifoliola Hayata subsp.urophylla%oleanolic acid%determination%RP-HPLC
目的 建立五指那藤中齐墩果酸的反相高效液相色谱含量测定方法.方法 色谱柱为LiChrospher C18柱(250 mm×4.6 mm,5μm),流动相为乙腈-甲醇-水-冰乙酸-三乙胺(65:12:23:0.04:0.02),流速1.0 mL/min,检测波长210 nm,柱温30 ℃.结果 齐墩果酸进样量在0.012 36~0.247 2μg范围内与峰面积呈良好的线性关系,回归方程为Y=5.13×105X-6.11×102(r=0.999 9);平均加样回收率为97.47%,RSD=2.35%(n=6).结论 该方法简便、准确、可靠,可作为五指那藤中齐墩果酸的含量测定方法.
目的 建立五指那籐中齊墩果痠的反相高效液相色譜含量測定方法.方法 色譜柱為LiChrospher C18柱(250 mm×4.6 mm,5μm),流動相為乙腈-甲醇-水-冰乙痠-三乙胺(65:12:23:0.04:0.02),流速1.0 mL/min,檢測波長210 nm,柱溫30 ℃.結果 齊墩果痠進樣量在0.012 36~0.247 2μg範圍內與峰麵積呈良好的線性關繫,迴歸方程為Y=5.13×105X-6.11×102(r=0.999 9);平均加樣迴收率為97.47%,RSD=2.35%(n=6).結論 該方法簡便、準確、可靠,可作為五指那籐中齊墩果痠的含量測定方法.
목적 건립오지나등중제돈과산적반상고효액상색보함량측정방법.방법 색보주위LiChrospher C18주(250 mm×4.6 mm,5μm),류동상위을정-갑순-수-빙을산-삼을알(65:12:23:0.04:0.02),류속1.0 mL/min,검측파장210 nm,주온30 ℃.결과 제돈과산진양량재0.012 36~0.247 2μg범위내여봉면적정량호적선성관계,회귀방정위Y=5.13×105X-6.11×102(r=0.999 9);평균가양회수솔위97.47%,RSD=2.35%(n=6).결론 해방법간편、준학、가고,가작위오지나등중제돈과산적함량측정방법.
Objective To establish an RP-HPLC method for content determination of oleanolic acid in Stauntonia obovatifoliola Hayata subsp.urophylla.Methods The RP-HPLC with LiChrospher C18 column (250 mm × 4.6 mm, 5μm) was used, acetonitrile-methonal-water-acetic acid-triethylamine (65:12:23:0.04:0.02) was used as mobile phase, with flow rate of 1.0 mL/min, detection wavelength at 210 nm and column temperature at 30℃.Results Oleanolic acid showed good linearity (r=0.999 9) in the range of 0.012 36-0.247 2μg;regressive equation wasY=5.13 × 105X - 6.11 × 102;the average recovery was 97.47%;RSD was 2.35% (n=6).Conclusion The method is simple, accurate and reliable, which can be used to determine the content of oleanolic acid in Stauntonia obovatifoliola Hayata subsp.urophylla.
