CAJ | 학술논문

目的建立复方沙塔干口服液的质量标准.方法采用薄层色谱法对该制剂中的君药川芎进行专属性鉴别;采用高效液相色谱法对川芎中有效成分阿魏酸进行含量测定.色谱柱:Diamonsil C18柱(4.6 mm×250 mm, 5μm);流动相:甲醇-1%冰醋酸,梯度洗脱(0~5 min,35%甲醇;5~8 min,35%→23%甲醇;8~22 min,23%甲醇);检测波长:322 nm;流速:1.0 mL/min;柱温:30 ℃;进样量:5μL.结果阿魏酸进样量在0.039 4~0.630 0μg范围内线性关系良好(r=0.999 7,n=7),平均加样回收率为98.22%,RSD=2.62%(n=6).结论本研究所建立的质量标准结果准确,灵敏度高,重复性良好,可用于复方沙塔干口服液的质量控制.
목적 건립복방사탑간구복액적질량표준.방법 채용박층색보법대해제제중적군약천궁진행전속성감별;채용고효액상색보법대천궁중유효성분아위산진행함량측정.색보주:Diamonsil C18주(4.6 mm×250 mm, 5μm);류동상:갑순-1%빙작산,제도세탈(0~5 min,35%갑순;5~8 min,35%→23%갑순;8~22 min,23%갑순);검측파장:322 nm;류속:1.0 mL/min;주온:30 ℃;진양량:5μL.결과 아위산진양량재0.039 4~0.630 0μg범위내선성관계량호(r=0.999 7,n=7),평균가양회수솔위98.22%,RSD=2.62%(n=6).결론 본연구소건립적질량표준결과준학,령민도고,중복성량호,가용우복방사탑간구복액적질량공제.
Objective To establish the quality standards for compound Shatagan Oral Liquid. Methods Chuanxiong Rhizoma was identified by TLC. The content of ferulic acid was determined by HPLC. Separation was performed on a Diamonsil C18 column (4.6 mm × 250 mm, 5μm) with a mobile phase consisting of methanol-1% acetic acid solution in gradient elution (0-5 min, 35% methanol;5-8 min, 35%→23% methanol;8-22 min, 23% methanol) at 30℃;The flow rate was 1.0 mL/min;The injection volume was 5μL;The detection wavelength was 322 nm.Results Ferulic acid showed a good linear relationship in the range of 0.039 4-0.630 0μg (r=0.999 7,n=7). The average recovery was 98.22% and RSD was 2.62% (n=6).Conclusion The method is reliable, sensitive and with repeatability, which can be used as the quality control method for compound Shatagan Oral Liquid.