CAJ | 학술논문

目的：评价细胞外基质蛋白含sushi重复蛋白X连锁2（SRPX2）对人脐静脉内皮细胞（HUVECs）血管生成能力的影响。方法分别用重组pcDNA3．1－SRPX2及空载质粒转染HEK293T细胞后收集条件培养基，培养HUVECs，实验分转染组、阴性对照组和空白对照组。 CCK－8试剂盒检测细胞增殖；Transwell迁移实验和划痕实验观察细胞的迁移能力；Matrigel基质胶体外管腔形成实验观察HUVECs管腔形成能力。结果3组间细胞增殖能力无明显差异。转染组管腔样结构分支点数为（97±4）个／视野，明显多于阴性对照组（57±3）和空白对照组（54±3）个／视野（P＜0．05）。转染组划痕6和12 h细胞迁移距离均明显大于阴性对照组和空白对照组，分别为（90±6）、（37±7）和（36±4）μm，（135±5）、（65±8）和（63±4）μm（P＜0．05）。 Transwell迁移实验中，转染组16 h迁移过膜细胞数为（549±10）个／视野，明显高于阴性对照组（334±11）和空白对照组（329±12）个／视野（P＜0．05）。结论 SRPX2可通过促进HUVECs的迁移及在Matrigel表面的管腔形成能力，增强其血管生成能力。
목적：평개세포외기질단백함sushi중복단백X련쇄2（SRPX2）대인제정맥내피세포（HUVECs）혈관생성능력적영향。방법분별용중조pcDNA3．1－SRPX2급공재질립전염HEK293T세포후수집조건배양기，배양HUVECs，실험분전염조、음성대조조화공백대조조。 CCK－8시제합검측세포증식；Transwell천이실험화화흔실험관찰세포적천이능력；Matrigel기질효체외관강형성실험관찰HUVECs관강형성능력。결과3조간세포증식능력무명현차이。전염조관강양결구분지점수위（97±4）개／시야，명현다우음성대조조（57±3）화공백대조조（54±3）개／시야（P＜0．05）。전염조화흔6화12 h세포천이거리균명현대우음성대조조화공백대조조，분별위（90±6）、（37±7）화（36±4）μm，（135±5）、（65±8）화（63±4）μm（P＜0．05）。 Transwell천이실험중，전염조16 h천이과막세포수위（549±10）개／시야，명현고우음성대조조（334±11）화공백대조조（329±12）개／시야（P＜0．05）。결론 SRPX2가통과촉진HUVECs적천이급재Matrigel표면적관강형성능력，증강기혈관생성능력。
Objective_To evaluate the proangiogenesis of extracelluar matrix proteins SRPX2 on HUVECs.Meth-ods_pcDNA3.1-SRPX2 vector ( transfection group) and pcDNA3.1 vector ( negative group) were transfected into HEK293T cells, then divided into 3 groups including blank group.The proliferation of HUVECs ( absorbance, A450 ) was detected by CCK-8 kit.The transmembrane cell number was counted by Transwell migration and wound healing assay to evaluate the migration ability of HUVECs.A three dimensional culture system of cells was construc-ted on the Matrigel, and tube formation number of HUVECs was assessed.Results_The proliferation of HUVECs ( absorbance,A450 ) among transfection group,negative group and blank group failed to show significant difference (P>0.05).A signifiant difference was noted in the total branch point of capillary tubes among transfection group, negative group and blank group[(97 ±4)/field versus (57 ±3) and (54 ±3)/field] (P<0.05).In wound healing <br> assay, the distance of transfection group compared to 0, 6 and 12 h were both significantly larger than that of nega-tive group and blank group [(90 ±6), (37 ±7),(36 ±4)μm and (135 ±5),(65 ±8),(63 ±4)μm respective-ly, both( P<0.05) ] .In Tranwell assay, the number of migrating cells in the transfection group was significantly more than negative group and blank group [(549 ±10)/field vs (334 ±11) and (329 ±12)/field(P<0.05)] af-ter co-culture 16 h.Conclusions_SRPX2 may promote angiogenesis by stimulating the migration and tubing on the Matrigel of HUVECs.