CAJ | 학술논문

目的：探讨IL－31对16HBE细胞VEGF、EGF和EGFR表达的影响及作用机制。方法 IL－31单独及分别联合P38 MAPK信号阻断剂SB203580或JNK信号阻断剂SP600125处理16HBE细胞，分别用real－time PCR和Western blot检测VEGF、EGF和EGFR mRNA及蛋白表达水平；采取Western blot分别检测P38 MAPK和JNK的磷酸化表达水平。结果与对照组相比，IL－31显著上调VEGF、EGF和EGFR mRNA和蛋白的表达（ P＜0．01），并且能显著上调P38 MAPK和JNK的磷酸化表达（P＜0．01）。与IL－31组相比，IL－31＋SB203580或SB203580组p－P38 MAPK的表达水平均明显下降（P＜0．01），而IL－31＋SP600125或SP600125组p－JNK的表达水平均明显下降（P＜0．01）。与IL－31组相比，IL－31联合SB203580或SP600125组VEGF的表达均明显下降（ P＜0．01），而EGF和EGFR的表达在IL－31联合SB203580组显著下降（P＜0．01）。结论 IL－31可通过激活P38 MAPK及JNK信号通路而诱导VEGF表达；而IL－31诱导的EGF和EGFR表达则主要通过P38 MAPK信号通路介导。
목적：탐토IL－31대16HBE세포VEGF、EGF화EGFR표체적영향급작용궤제。방법 IL－31단독급분별연합P38 MAPK신호조단제SB203580혹JNK신호조단제SP600125처리16HBE세포，분별용real－time PCR화Western blot검측VEGF、EGF화EGFR mRNA급단백표체수평；채취Western blot분별검측P38 MAPK화JNK적린산화표체수평。결과여대조조상비，IL－31현저상조VEGF、EGF화EGFR mRNA화단백적표체（ P＜0．01），병차능현저상조P38 MAPK화JNK적린산화표체（P＜0．01）。여IL－31조상비，IL－31＋SB203580혹SB203580조p－P38 MAPK적표체수평균명현하강（P＜0．01），이IL－31＋SP600125혹SP600125조p－JNK적표체수평균명현하강（P＜0．01）。여IL－31조상비，IL－31연합SB203580혹SP600125조VEGF적표체균명현하강（ P＜0．01），이EGF화EGFR적표체재IL－31연합SB203580조현저하강（P＜0．01）。결론 IL－31가통과격활P38 MAPK급JNK신호통로이유도VEGF표체；이IL－31유도적EGF화EGFR표체칙주요통과P38 MAPK신호통로개도。
Objective_To explore the influence and mechanism of IL-31 on the expression of VEGF, EGF and EG-FR in 16HBE cells.Methods_16HBE cells were cultured and treated with IL-31 with or without SB203580 or SP600125, real-time PCR and Western blot were applied to determine the mRNA and protein expression of VEGF, EGF and EGFR respectively.Meanwhile, Western blot was used to examine the changes of P38 MAPK and JNK signaling pathways.Results_Compared with control group, the mRNA expression of VEGF, EGF and EGFR was increased markedly under the stimulation of IL-31 ( P<0.01 ) , the expression of p-P38 MAPK and p-JNK signifi-cantly increased ( P<0.01) .Compared with IL-31 group, the expression of p-P38 MAPK significantly decreased in IL-31 combined with SB203580 or SB203580 group ( P <0.01 ) , while the expression of p-JNK markedly decreased in IL-31 combined with SP600125 or SP600125 group( P<0.01) .Compared with IL-31 group, the expression of VEGF was significantly decreased in IL-31 combined with SB203580 or SP600125 group ( P <0.01 ) , while the expression of EGF and EGFR was markedly declined in IL-31 combined with SB203580 group ( P<0.01 ) .Conclusions_IL-31 may up-regulate the expression of VEGF through activating P38 MAPK and JNK signaling pathways and up-regulate the expression of EGF and EGFR through activating P38 MAPK signaling path-way in16 HBE cells.